通过泗水传统市场的MDT染色方法检测流浪猫的血液寄生虫

Intan Permatasari Hermawan, D. Sari, Mohammad Rio Noor Rahman
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引用次数: 0

摘要

本研究的目的是用MDT染色法检测流浪猫的血液寄生虫。从泗水4个市场抽取40份血样,每个市场抽取10份流浪猫血样。从头前静脉或隐静脉取血三(3)毫升。然后用MDT染色,在1000倍显微镜下检查。结果40份样品中无原体sp阳性7份(17.5%)。本研究需要采用PCR、抗体检测(IFA试验)、ELISA和western免疫印迹法进行高级诊断,尽管该试剂可检测血液染色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DETEKSI PARASIT DARAH PADA KUCING LIAR (STRAY CATS) DENGAN METODE PEWARNAAN MDT DI PASAR TRADISIONAL SURABAYA
The aim of this study was to detect blood parasites of stray cats with an MDT staining test. Total samples were 40 blood taken from 4 markets in Surabaya, every market took 10 samples of stray cats. Three (3) cc Blood took from vena cephalica antebrachial anterior or vena saphena. Then blood samples were stained using MDT and checked under microscope 1000 magnification. The result showed 7 positive (17,5%) Anaplasma sp from 40 samples. This study needs advanced diagnosis using PCR, antibody detection (IFA test), ELISA, and western immunoblot assay although blood staining was detected by the agent.
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