同一天痰涂片镜检与经济染色步骤诊断肺结核

The Journal of Clinical and Scientific Research Pub Date : 2015-07-01 DOI:10.15380/2277-5706.JCSR.15.019

T. Chandra, R. Selvaraj, Y. Sharma

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引用次数: 2

摘要

背景:采用改良Ziehl - Neelsen (MZN)染色法和瓶法对上午现场法(SM)和当天法(SS2)采集的痰液标本进行抗酸杆菌(AFB)阳性研究。方法:采集252例患者的3份标本(点、次、晨)，制作重复涂片，用MZN染色法和瓶法进行染色。结果:SM法MZN染色阳性34例(13.5%)，罐子法阳性33例(13%)(p>0.99);SS2法MZN染色34例(13.5%)，罐法32例(12.7%)(p>0.99)。结论:罐法成本较低，诊断率相近，可用于临床培训。

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Same day sputum smear microscopy with an economical destaining step for the diagnosis of pulmonary tuberculosis

Back ground: Acid fast bacilli (AFB) positivity using modified Ziehl Neelsen (MZN) staining and jar method was studied in sputum samples obtained by spot morning (SM) and same day (SS2) approaches. Methods: Three specimens (spot, second spot and morning) were collected in 252 patients, duplicate smears were prepared and stained by MZN staining and jar method. Results: Smear positivity was 34 (13.5%) and 33 (13%) for MZN staining and jar method respectively (p>0.99) for the SM approach; and 34 (13.5%) and 32 (12.7%) for MZN staining and jar method (p>0.99) respectively for SS2 approach. Conclusion: Jar method in view of lesser cost and similar diagnostic yielf can be useful for training purposes.

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The Journal of Clinical and Scientific Research

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