Ryosuke Yuchi, Jiafeng Yao, Takeomi Mizutani, H. Obara, M. Sugawara, M. Takei
{"title":"三维多层微通道中交流电场作用下细胞动态运动分析","authors":"Ryosuke Yuchi, Jiafeng Yao, Takeomi Mizutani, H. Obara, M. Sugawara, M. Takei","doi":"10.1109/MHS.2015.7438285","DOIUrl":null,"url":null,"abstract":"In the field of regenerative medicine, it is expected to separate disease-causing cells from healthy cells by electrical method with label-free and high efficiency. The purpose of this study is to find the differences of speeds between disease-causing cells and healthy cells under alternating current electric field in 3D multi-layered microchannel. We prepared intracellular charge-differed two cell types: wild type cells (WT) and wild type cells with green fluorescent protein (GFP)-histone (GFP-HT). The cell dynamic movement of each type was detected by cell tracking. We found a difference between WT velocity and GFP-HT velocity around supplying electrode and discussed by particle tracking velocimetry. The present results provide a great significance for separation of disease-causing cells from healthy cells in the microchannel.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"62 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of dynamic cell movement under AC electric field in 3D multi-layered microchannel\",\"authors\":\"Ryosuke Yuchi, Jiafeng Yao, Takeomi Mizutani, H. Obara, M. Sugawara, M. Takei\",\"doi\":\"10.1109/MHS.2015.7438285\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the field of regenerative medicine, it is expected to separate disease-causing cells from healthy cells by electrical method with label-free and high efficiency. The purpose of this study is to find the differences of speeds between disease-causing cells and healthy cells under alternating current electric field in 3D multi-layered microchannel. We prepared intracellular charge-differed two cell types: wild type cells (WT) and wild type cells with green fluorescent protein (GFP)-histone (GFP-HT). The cell dynamic movement of each type was detected by cell tracking. We found a difference between WT velocity and GFP-HT velocity around supplying electrode and discussed by particle tracking velocimetry. The present results provide a great significance for separation of disease-causing cells from healthy cells in the microchannel.\",\"PeriodicalId\":165544,\"journal\":{\"name\":\"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)\",\"volume\":\"62 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/MHS.2015.7438285\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MHS.2015.7438285","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analysis of dynamic cell movement under AC electric field in 3D multi-layered microchannel
In the field of regenerative medicine, it is expected to separate disease-causing cells from healthy cells by electrical method with label-free and high efficiency. The purpose of this study is to find the differences of speeds between disease-causing cells and healthy cells under alternating current electric field in 3D multi-layered microchannel. We prepared intracellular charge-differed two cell types: wild type cells (WT) and wild type cells with green fluorescent protein (GFP)-histone (GFP-HT). The cell dynamic movement of each type was detected by cell tracking. We found a difference between WT velocity and GFP-HT velocity around supplying electrode and discussed by particle tracking velocimetry. The present results provide a great significance for separation of disease-causing cells from healthy cells in the microchannel.