三维多层微通道中交流电场作用下细胞动态运动分析

Ryosuke Yuchi, Jiafeng Yao, Takeomi Mizutani, H. Obara, M. Sugawara, M. Takei
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引用次数: 0

摘要

在再生医学领域,有望实现无标记、高效率的电分离致病细胞和健康细胞。本研究的目的是寻找在交变电场作用下三维多层微通道中致病细胞和健康细胞的速度差异。我们制备了两种细胞类型:野生型细胞(WT)和野生型细胞(GFP-组蛋白)。通过细胞跟踪检测各类型细胞的动态运动。我们发现了WT速度和GFP-HT速度在供电电极周围的差异,并通过粒子跟踪速度法进行了讨论。本研究结果对微通道中致病细胞与健康细胞的分离具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of dynamic cell movement under AC electric field in 3D multi-layered microchannel
In the field of regenerative medicine, it is expected to separate disease-causing cells from healthy cells by electrical method with label-free and high efficiency. The purpose of this study is to find the differences of speeds between disease-causing cells and healthy cells under alternating current electric field in 3D multi-layered microchannel. We prepared intracellular charge-differed two cell types: wild type cells (WT) and wild type cells with green fluorescent protein (GFP)-histone (GFP-HT). The cell dynamic movement of each type was detected by cell tracking. We found a difference between WT velocity and GFP-HT velocity around supplying electrode and discussed by particle tracking velocimetry. The present results provide a great significance for separation of disease-causing cells from healthy cells in the microchannel.
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