Quality Assessment of Platelet Concentrates Prepared after Whole Blood Overnight Storage

The present study focuses to assess the in vitro effect on quality of platelet concentrates (PCs) prepared from overnight-held whole blood (WB). Method: Thirty four units of WB were stored overnight at 20 to 24oC and processed on the following day, and 30 units of WB were processed immediately. Result: The volume of PCs from overnight-held WB on Day 1 was significantly lower. On the same day, the PCs were significantly lower in platelet count but by Day 3 and Day 5, the difference was disappeared. PCs made from overnight-held WB had a significantly higher total red blood count on Day 1, Day 3 and Day 5, and for total white blood count was significantly higher on Day 5. pH was significantly lower on Day 1, Day 3 and Day 5. Sterility test for both groups of PCs give negative results. Conclusion: PRP-derived PCs from overnight-held WB have significantly different in vitro variables as from freshly processed WB. However, based on one-sample t-test comparison with the quality requirement of PCs, the use of the PCs from overnight-held WB was possible as the results of volume, platelet count and pH were significantly higher than the quality requirement and specification. 