The black rice ethanol extract effect on polymorphonuclear cells proliferation of rats induced by lipopolysaccharide

Inflammation is a defense that exists in the body when an infection occurs. Excessive inflammatory reactions can be induced by lipopolysaccharides (LPS) which are the most immunogenic components of a bacterial capsule or cell wall, which will result in damaging effects on the body. Black rice (Oryza sativa L.indica) is one of the natural ingredients that contains phenols, flavonoids, and antioxidant anthocyanin as immunomodulator. This study aims to find out the effect of giving black rice ethanol extract as an antiinflammatory to the proliferation of polymorphonuclear cells (PMN) in the spleen of rats induced on lipopolysaccharides. This study was conducted using 27 mice who were given black rice ethanol extract as much as 400 mg/kg BW in 14 days and then rats induced by LPS 0.3 mg/kg BW at 24 hours intraperitoneally before being CO2 euthanized and taken the spleen to be checked for measuring the size of the germinal center diameter. The observations showed the results of the germinal center diameter size in the treatment group with a value of 1,311 ± 0.226 μm (p<0.05 compared to positive control group which showed that LPS administration led to the disorganization of the white pulp and the reduction of lymphocyte cells and PMN in the germinal center. In conclusion, black rice ethanol extract at a dose of 400 mg/kgBB can affect the proliferation of PMN indicated by changes in the morphology of the white pulp in the germinal center in the spleen of rats.