Tempeh and Vital Wheat Gluten based Analog Meat Development as Vegetarian Alternative Food

Vitamin B12 deficiency is one of the main problems in the vegetarian diet with prevalency from 11% to 90%. Tempeh and vital wheat gluten-based meat analog are expected to provide vitamin B12 for vegetarians. Completely Randomized Design was used as the experimental design. The organoleptic test was carried out by 25 semi-trained panelists and 30 consumers. The content of vitamin B12, protein, fat, carbohydrate, moisture, ash, Total Plate Count, and also production cost was analyzed. Organoleptic test result showed that F2 has the best acceptability with 6,67 mg/100g of vitamin B12, 9,25% protein, 2,94% fat, 28,32% carbohydrate, 56,72% moisture. 2,69% ash, and 2,3 x 104 cfu/g in Total Plate Count. The cost to produce one package of tempeh and vital gluten wheat-based meat analog is 9.171 rupiahs. Therefore, it is proved that tempeh and vital wheat gluten can produce a meat analog with high vitamin B12 content that also fulfills the minimum quality requirements in SNI 3818:2014. However, it was necessary to analyze the storability of the product, also analyze the protein content with the Kjeldahl method and analyze the pseudo vitamin B12 that might be contained in the product.