骆驼副结核:ELISA和PCR的诊断效果

Ameen M. Alhebabi, A. Alluwaimi
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引用次数: 18

摘要

沙特阿拉伯的骆驼副结核病有广泛的文献记载。采用酶联免疫吸附试验(ELISA)和聚合酶链反应(PCR)两种方法对沙特阿拉伯地区MAP感染进行控制。采用反刍动物酶联免疫吸附试验对1 ~ 4岁(幼年)、5 ~ 9岁(中年)和10 ~ 15岁(老年)3个不同年龄组的861份血清样本进行筛选。ELISA阳性29例,其中14例为低龄组。PCR分析主要针对低龄组收集的粪便样本和其他组所有ELISA阳性样本进行。在310个检测样本中,PCR仅检测出97个阳性样本。在年轻年龄组中,PCR检测到的阳性样本多于ELISA(占276个年轻样本的29%)。结果表明,ELISA可有效筛选骆驼MAP感染;然而,它在年轻群体中的敏感性令人怀疑。另一方面,由于MAP脱落模式的巨大差异,PCR只能作为ELISA的辅助测试有效。因此,ELISA和PCR对MAP感染的控制是可行的,但ELISA在早期发现感染时不够谨慎。尽管潜伏期超过10周,但分离骆驼MAP进行基因分型的尝试仍未成功。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Paratuberculosis in Camel (Camelus dromedarius): The Diagnostic Efficiency of ELISA and PCR
The camel paratuberculosis in Saudi Arabia was widely documented. ELISA and PCR were employed to exploit their feasibility for control of the MAP infection in Saudi Arabia. Ruminant ELISA test was used for the screening of 861 serum samples that were collected from three different age groups, 1-4 years (young age), 5-9 years (middle age) and 10-15 years (old age). Only 29 samples were revealed as ELISA positive in which 14 samples were from the young age group. PCR analysis was carried out mainly on the fecal samples that were collected from young age group and all the ELISA positive samples of other groups. PCR detected only 97 positive samples out of 310 tested samples. PCR detected more positive samples in the young age group than ELISA (29% of 276 young age samples). The ELISA proved effective as a screening tool for MAP infection in camel; however its sensitivity in the young group is skeptical. PCR on the other hand, is effective only as supportive test to the ELISA due to the great variations in the pattern of MAP shedding. Hence, ELISA and PCR were shown feasible for control of the MAP infection, but ELISA was not prudent in the early detection of the infection. Attempt to isolate the camel MAP for its genotyping was not successful despite the incubation for more than 10 weeks.
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