日本参SS基因表达载体的构建与转化。

L. Zhang, T. T. Wang
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引用次数: 0

摘要

参是五加科人参属多年生草本植物,是世界七大珍稀濒危中药材之一。通过克隆SS片段,构建了表达载体pCXSN-PjSS和pCXSN-antiPjSS,并将其导入工程菌LBA4404农杆菌中。聚合酶链反应(PCR)表明,PjSS、antiPjSS和Hyg在烟草中得到整合。逆转录pcr结果表明,PjSS在烟草中被转录成mRNA并高表达,而抗PjSS不表达。人参皂苷Re含量检测结果表明,转基因烟草可提高人参皂苷Re含量,而抗转基因烟草可降低人参皂苷Re含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Construction and transformation of expression vector containing Panax japonicus SS gene.
Panax japonicus C.A. Meyer, a perennial herb belonging to the Araliaceae ginseng genus, is one of the seven rare and endangered Chinese medical herbs. By cloning the SS segment, the expression vectors pCXSN-PjSS and pCXSN-antiPjSS were constructed and introduced into Agrobactria LBA4404, which is used for engineering bacteria. Polymerase chain reaction (PCR) showed that the PjSS, antiPjSS, and Hyg were integrated in Nicotiana tabacum. Reverse transcription-PCR indicated that the PjSS was transcribed into mRNA in N. tabacum and was highly expressed, while the antiPjSS was not expressed. Detection of Ginsenoside Re content showed that transgenetic N. tabacum can increase the content of Ginsenoside Re and that anti-transgenetic N. tabacum decreased the content of Ginsenoside Re.
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