黄曲霉产纤维素酶的稳定性规律

Okonkwo If
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引用次数: 1

摘要

考虑到温度对真菌纤维素酶活性的影响较大,本试验旨在研究腐木黄曲霉纤维素酶的稳定性规律。采用羧甲基纤维素、滤纸和棉絮法对纯化后的黄曲霉纤维素酶进行了表征。最高温度为90℃,最适温度为70℃。酶在100℃下保存1小时,酶的活性保持在50%以上,在80℃下保存1小时,酶的活性保持在80%。酶的衰变研究表明,CMC和滤纸在0小时的纤维素酶活性都保持在100%。然而,随着孵育时间的增加,活性下降,但在40-100°C范围内,酶在两种底物中1h仍保持50%以上的活性。研究表明,纯化的黄曲霉纤维素酶显示出有用的特性,其中包括;高最佳温度,增加热稳定性。它们还提供了额外的优势,因为它们比中位酶更坚硬,而且更耐热变性和化学变性。因此,它们是开发商业生物技术和研究蛋白质稳定性的重要工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Stability Pattern of the Cellulase Enzyme Produced by Aspergillus flavus
In view of the fact that temperature plays key role in the activity of fungal cellulases, this experiment was designed to assess the stability pattern of the cellulase Enzyme produced by Aspergillus flavus derived from rotten wood. The characterization of the purified cellulase enzyme of Aspergillus flavus was carried out using Carboxymethyl cellulose, Filter paper and Cotton wool assays. The maximum and optimum temperatures were 90°C and 70°C, respectively. The enzyme retained above 50% of its original activity at 100°C for 1h and up to 80% at 80°C for 1h Enzyme decay study showed that Cellulase activity remained 100 % at 0 h for both CMC and filter paper. However, as incubation period increased, the activity decreased, but the enzyme still retained above 50 % activity in both substrates at 1h, over the range of 40-100°C. The study carried out showed that the purified Aspergillus flavus cellulases demonstrated useful properties among which include; high optimum temperature, increased thermal stability. They also offer additional advantage as they are more rigid than meso enzymes and moreover, are more resistant to thermal and chemical denaturation. Hence are remarkable tools for developing commercial biotechnologies and for studying protein stability.
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