Safoura Seifi , Tahere Padeganeh , Syed Razie Rezaee , Ali Bijani
{"title":"诱导型一氧化氮合酶(iNOS)在牙滤泡、滤泡囊肿和钙化囊性牙源性肿瘤中的表达的免疫组化评价","authors":"Safoura Seifi , Tahere Padeganeh , Syed Razie Rezaee , Ali Bijani","doi":"10.1016/j.ijdsr.2015.11.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p><span><span>The calcifying cystic odontogenic tumor (CCOT) is a benign cystic neoplasm<span> with a variety of histopathological and clinical features in comparison with the follicular cyst. </span></span>iNOS<span> is an enzyme that produces free radicals and is a mediator regulator of the inflammatory response. It has been implicated in tumorigenesis as well. The aim of this study was to compare iNOS expression in the </span></span>dental follicle and two odontogenic lesions with different aggressive behaviors.</p></div><div><h3>Methods</h3><p>In this cross-sectional study, 44 paraffin blocks were selected from the dental follicle, follicular cyst, and CCOT, and immunohistochemical staining was done by iNOS. The percentage and intensity of staining (total score) were calculated in the cytoplasm of the epithelial cells.</p></div><div><h3>Results</h3><p>The highest total score of iNOS staining was found in the cytoplasm of the epithelial cells of CCOT when compared to the follicular cyst and dental follicle (<em>p</em> <!-->=<!--> <!-->0.000). There was a significant difference in the final score between the follicular cyst and dental follicle (<em>p</em> <!-->=<!--> <!-->0.001).</p></div><div><h3>Discussion</h3><p>The overexpression of iNOS in the CCOTs plays a role in its pathogenesis and iNOS, because of producing free radicals and damaging the oral tissue, may contribute to more aggressive behaviors of CCOT.</p></div>","PeriodicalId":100695,"journal":{"name":"International Journal of Dental Science and Research","volume":"3 1","pages":"Pages 9-14"},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijdsr.2015.11.007","citationCount":"0","resultStr":"{\"title\":\"Immunohistochemical evaluation of inducible nitric oxide synthase (iNOS) expression in the dental follicle, follicular cyst, and calcifying cystic odontogenic tumor\",\"authors\":\"Safoura Seifi , Tahere Padeganeh , Syed Razie Rezaee , Ali Bijani\",\"doi\":\"10.1016/j.ijdsr.2015.11.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p><span><span>The calcifying cystic odontogenic tumor (CCOT) is a benign cystic neoplasm<span> with a variety of histopathological and clinical features in comparison with the follicular cyst. </span></span>iNOS<span> is an enzyme that produces free radicals and is a mediator regulator of the inflammatory response. It has been implicated in tumorigenesis as well. The aim of this study was to compare iNOS expression in the </span></span>dental follicle and two odontogenic lesions with different aggressive behaviors.</p></div><div><h3>Methods</h3><p>In this cross-sectional study, 44 paraffin blocks were selected from the dental follicle, follicular cyst, and CCOT, and immunohistochemical staining was done by iNOS. The percentage and intensity of staining (total score) were calculated in the cytoplasm of the epithelial cells.</p></div><div><h3>Results</h3><p>The highest total score of iNOS staining was found in the cytoplasm of the epithelial cells of CCOT when compared to the follicular cyst and dental follicle (<em>p</em> <!-->=<!--> <!-->0.000). There was a significant difference in the final score between the follicular cyst and dental follicle (<em>p</em> <!-->=<!--> <!-->0.001).</p></div><div><h3>Discussion</h3><p>The overexpression of iNOS in the CCOTs plays a role in its pathogenesis and iNOS, because of producing free radicals and damaging the oral tissue, may contribute to more aggressive behaviors of CCOT.</p></div>\",\"PeriodicalId\":100695,\"journal\":{\"name\":\"International Journal of Dental Science and Research\",\"volume\":\"3 1\",\"pages\":\"Pages 9-14\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ijdsr.2015.11.007\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Dental Science and Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213997415000373\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Dental Science and Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213997415000373","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Immunohistochemical evaluation of inducible nitric oxide synthase (iNOS) expression in the dental follicle, follicular cyst, and calcifying cystic odontogenic tumor
Objective
The calcifying cystic odontogenic tumor (CCOT) is a benign cystic neoplasm with a variety of histopathological and clinical features in comparison with the follicular cyst. iNOS is an enzyme that produces free radicals and is a mediator regulator of the inflammatory response. It has been implicated in tumorigenesis as well. The aim of this study was to compare iNOS expression in the dental follicle and two odontogenic lesions with different aggressive behaviors.
Methods
In this cross-sectional study, 44 paraffin blocks were selected from the dental follicle, follicular cyst, and CCOT, and immunohistochemical staining was done by iNOS. The percentage and intensity of staining (total score) were calculated in the cytoplasm of the epithelial cells.
Results
The highest total score of iNOS staining was found in the cytoplasm of the epithelial cells of CCOT when compared to the follicular cyst and dental follicle (p = 0.000). There was a significant difference in the final score between the follicular cyst and dental follicle (p = 0.001).
Discussion
The overexpression of iNOS in the CCOTs plays a role in its pathogenesis and iNOS, because of producing free radicals and damaging the oral tissue, may contribute to more aggressive behaviors of CCOT.
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