Ameliorative Effect of Vanillin against Propiconazole Induced Hepatorenal and Testicular Injury in Male Albino Rats

Vanillin commonly used in pharmaceutical industry, food and beverage processing, and in cosmetics and perfumes preparation. To better understand the effects of vanillin in reduction of tissue injuries resulted from Fungicides misuses. Fungicides like propiconazole are used extensively in greenhouse nowadays. The hazard effects of mycotoxin occurrence affect all defense and immunocytes of animals and reduced its production. But safety of use of fungicide and develop of new generation of fungicide often take a focus from researchers and authority. Propiconazole treatments at different doses have no effect on body weight. Notably, vanillin treatment reduced toxic effects of propiconazole induced in male rats’ evidence by liver and kidney biomarkers and improvement level of antioxidants level. Also, vanillin restored tissue architecture nearly similar to control group. Taken collectively, vanillin had a protective role against propiconazole induced toxicity. Ameliorative Effect of Vanillin against Propiconazole Induced Hepatorenal and Testicular Injury in Male Albino Rats ARC Journal of Animal and Veterinary Sciences Page | 13 2. MATERIALS AND METHODS 2.1. Tested Chemical propiconazole was obtain from institute for pesticide residues analysis in Doky, ministry of agriculture, Egypt. Vanillin was purchase from sigma Cairo, Egypt 2.2. Laboratory Animals 40 Mature males’ albino rats were obtained from Experimental Unit in the Faculty of Pharmacy, Mansoura University; Animals were weighing about 100 ±10 gm and obviously healthy then were grouping and housing in plastic cages with soft wood shavings as a bedding material that change adequately to ensures a low level of ammonia and to keep animals clean and dry. Animals kept for 2 weeks for accommodation and maintained on a balanced ration before the start of the experiment. Also, standard laboratory pelleted diet and water WERE received ad libitium throughout the experiment and Light cycles of 12 hours’ light to 12 hours dark seem to be adequate in order to promote rodents' breeding. 2.3. Experimental Design for Toxicity Propiconazole and the Effects of Vanillin Curing Propiconazole Induced Injuries in Male Albino Rats Forty males were separated into five groups with eight rats per group. The first group was received 0.5 ml distilled water and used as control; the second group was administered 150 mg/kg(6), 1/10 LD50), of propiconazole, the third group was gavaged 75 mg/kg (6), 1/20 LD50, of propiconazole, the fourth group was treated with 1/10 LD50 of propiconazole and was give 150 mg/kg of vanillin intraperitoneally, the fifth group was treated with 1/20 of propiconazole and was give 150 mg/kg of vanillin intraperitoneally. Both fungicide and vanillin were received twice per week for 6 weeks. The protocol was approved by Institutional Research Board of faculty of medicine, Mansoura university, Egypt (R.20.07.924) and study was done with agreement with ethical guidelines and the rest of sacrificed rats was discarded safety.