J. Agrizzi, A. C. Loss, A. Farro, R. Duda, L. P. Costa, Y. Leite
{"title":"大西洋森林哺乳动物线粒体DNA序列的分子诊断:双翅目有袋动物","authors":"J. Agrizzi, A. C. Loss, A. Farro, R. Duda, L. P. Costa, Y. Leite","doi":"10.2174/1874336601205010002","DOIUrl":null,"url":null,"abstract":"Most living species of New World marsupials are classified in the family Didelphidae, with 98 species and 18 genera currently recognized. We sequenced fragments of two mitochondrial genes of didelphid marsupials from the At- lantic Forest of eastern South America, a biodiversity hotspot. We evaluated sequence divergences within and among spe- cies and contrasted the efficiency of cytochrome c oxidase subunit I (COI) with cytochrome b (CytB) in species-level di- agnosis. The average intraspecific genetic divergence of COI and CytB was 2.0% and 1.9%, respectively; which was about five times lower than the comparison among species of the same genus (11.2 and 10.8%). In both genes, divergence levels among closely related species are usually higher than within species. The barcoding gap is similar in COI and CytB, indicating that either gene can be used in molecular diagnoses of didelphid species. DNA barcodes are a welcome addition to traditional taxonomic methods when viewed as additional diagnostic characters in the context of integrative taxonomy.","PeriodicalId":404991,"journal":{"name":"The Open Zoology Journal","volume":"28 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2012-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"23","resultStr":"{\"title\":\"Molecular Diagnosis of Atlantic Forest Mammals Using Mitochondrial DNA Sequences: Didelphid Marsupials\",\"authors\":\"J. Agrizzi, A. C. Loss, A. Farro, R. Duda, L. P. Costa, Y. Leite\",\"doi\":\"10.2174/1874336601205010002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Most living species of New World marsupials are classified in the family Didelphidae, with 98 species and 18 genera currently recognized. We sequenced fragments of two mitochondrial genes of didelphid marsupials from the At- lantic Forest of eastern South America, a biodiversity hotspot. We evaluated sequence divergences within and among spe- cies and contrasted the efficiency of cytochrome c oxidase subunit I (COI) with cytochrome b (CytB) in species-level di- agnosis. The average intraspecific genetic divergence of COI and CytB was 2.0% and 1.9%, respectively; which was about five times lower than the comparison among species of the same genus (11.2 and 10.8%). In both genes, divergence levels among closely related species are usually higher than within species. The barcoding gap is similar in COI and CytB, indicating that either gene can be used in molecular diagnoses of didelphid species. DNA barcodes are a welcome addition to traditional taxonomic methods when viewed as additional diagnostic characters in the context of integrative taxonomy.\",\"PeriodicalId\":404991,\"journal\":{\"name\":\"The Open Zoology Journal\",\"volume\":\"28 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-01-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"23\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Open Zoology Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874336601205010002\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Zoology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874336601205010002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular Diagnosis of Atlantic Forest Mammals Using Mitochondrial DNA Sequences: Didelphid Marsupials
Most living species of New World marsupials are classified in the family Didelphidae, with 98 species and 18 genera currently recognized. We sequenced fragments of two mitochondrial genes of didelphid marsupials from the At- lantic Forest of eastern South America, a biodiversity hotspot. We evaluated sequence divergences within and among spe- cies and contrasted the efficiency of cytochrome c oxidase subunit I (COI) with cytochrome b (CytB) in species-level di- agnosis. The average intraspecific genetic divergence of COI and CytB was 2.0% and 1.9%, respectively; which was about five times lower than the comparison among species of the same genus (11.2 and 10.8%). In both genes, divergence levels among closely related species are usually higher than within species. The barcoding gap is similar in COI and CytB, indicating that either gene can be used in molecular diagnoses of didelphid species. DNA barcodes are a welcome addition to traditional taxonomic methods when viewed as additional diagnostic characters in the context of integrative taxonomy.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
