用QuEChERS法定量测定选定蔬菜中农药残留

Meragal Kulathilakage Lasanthi Kumari Rajapakse, N. S. Weerakkody, Pulippu Widana Yoshida Lakshani
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引用次数: 2

摘要

水果和蔬菜中的农药残留已成为斯里兰卡的一个主要问题。因此,有必要对快速、简便、廉价、有效、坚固、安全(QuEChERS)的果蔬农药残留检测方法进行优化,确保食品安全。本研究旨在优化多残留农药分析方法,建立检测农药浓度的校准曲线,并测定putalum、Dambulla和Nuwara Eliya地区当地蔬菜番茄、白菜和辣椒中5种农药残留的流行率。采用多残留提取(QuEChERS法)气相色谱-质谱法测定农药残留。采用Diazinon、chlorpyrifos、Fipronil、Prothiofos和Tebuconazole 5种农药对QuEChERS方法进行了验证,其停留时间(min)分别为15.948、19.566、20.342、22.308和26.201。所有标准农药的检测系数均接近0.99,验证了检测方法的准确性。在45份蔬菜样本中,检出15份农药残留为毒死蜱、虫硫磷或戊唑唑。然而,所有被测农药的残留值均低于最高残留水平。在Matale和Puttlum地区采集的番茄和白菜中检出最多的农药残留是戊康唑,分别为0.128 ppm和0.052 ppm。因此,在获取农民使用的农药种类、收获间隔、农药使用频率和施用量等详细信息的同时,收集样本是很重要的,以供进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification of Pesticide Residues in Selected Vegetables using the QuEChERS Method
Pesticide residues in fruits and vegetables have become a major problem in Sri Lanka. Therefore, there is a need to optimize the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method to determine pesticide residues in fruits and vegetables and assure the safety of food. The objectives of this study were to optimize the method for multi-residue pesticide analysis, to develop calibration curve to detect concentration of pesticides and to determine prevalence of five pesticide residues in locally grown vegetables tomato, cabbage and capsicum in Puttalum, Dambulla and Nuwara Eliya districts. Pesticide residues were determined by Gas Chromatography with Mass Spectrometry after multi residue extraction procedure (QuEChERS method). The QuEChERS method was validated using five pesticides named Diazinon, Chlopyrifos, Fipronil, Prothiofos and Tebuconazole and their retention times in minutes were15.948, 19.566, 20.342, 22.308, and 26.201 respectively. Coefficient of detection was obtained near 0.99 for all tested standard pesticides confirming the accuracy of the test method. Out of 45 vegetables samples, 15 samples were detected with pesticide residues, either Chlopyrifos, Prothiofos or Tebuconazole. However, pesticide residual values were less than Maximum Residual Levels for all the tested pesticides. Tebuconazole was the mostly detected pesticide residue with 0.128 ppm and 0.052 ppm in tomato and cabbage collected from Matale and Puttlum districts respectively. Therefore, it is important to collect samples while obtaining farmer details including the type of pesticide applied, harvesting interval and frequency and application rate of pesticide for further study.
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