成骨细胞对Al2O3-Ti复合材料作为骨植入材料的反应

M. Bahraminasab, S. Arab, S. Ghaffari
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摘要

氧化铝-钛(Al2O3-Ti)复合材料具有增强的机械性能和腐蚀性能,最近在骨科和硬组织替代方面有了潜在的应用。然而,在任何临床应用之前,必须检查它们与生物环境的相互作用。方法:因此,本研究的目的是评估三种Al2O3-Ti复合材料的生物相容性,钛的体积百分比分别为25%、50%和75%。采用火花等离子烧结(SPS)法制备材料,将MC3T3-E1细胞培养于样品盘上,观察细胞活力、增殖、分化、矿化和粘附情况。进一步分析了复合材料的磷灰石形成能力和润湿性。采用SPS法制备了纯Ti (100Ti)和单片Al2O3 (0Ti)复合材料,并对其生物学特性进行了比较。结果:与100Ti(42.7%)相比,75Ti(95.0%)、50Ti(87.3%)和25Ti(63.9%)的细胞存活率均显著提高。纯Al2O3也能提高细胞存活率(89.9%)。此外,50Ti处理的细胞在早期具有较高的增殖能力,而75Ti处理的细胞在后期增殖能力较强。各组间细胞分化基本相等,且随时间延长而增加。复合材料表面基质矿化程度高于0Ti和100Ti表面。细胞在不同生物材料表面的粘附也不同,在100Ti表面以纺锤形细胞为主,在75Ti表面以树突状细胞和早期伪足细胞轻微增大,在0Ti、25Ti和50Ti表面以长树突延伸细胞为主。EDS分析结果表明,在SBF中浸泡20天后,Ca和P均在所有材料表面沉积。结论:我们的体外实验结果表明,75Ti、50Ti和25Ti复合材料作为承重骨科材料具有很高的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Osteoblastic cell response to Al2O3-Ti composites as bone implant materials
Introduction: Alumina-titanium (Al2O3-Ti) composites with enhanced mechanical and corrosion properties have been recently developed for potential applications in orthopaedics and hard tissue replacements. However, before any clinical use, their interactions with biological environment must be examined. Methods: The aim of this study, therefore, was to assess the biocompatibility of three Al2O3-Ti composites having 25, 50, and 75 volume percentages of titanium. These materials were made by spark plasma sintering (SPS), and MC3T3-E1 cells were cultured onto the sample discs to evaluate the cell viability, proliferation, differentiation, mineralization, and adhesion. Furthermore, the apatite formation ability and wettability of the composites were analysed. Pure Ti (100Ti) and monolithic Al2O3 (0Ti) were also fabricated by SPS and biological characteristics of the composites were compared with them. Results: The results showed that cell viability to 75Ti (95.0%), 50Ti (87.3%), and 25Ti (63.9%) was superior when compared with 100Ti (42.7%). Pure Al2O3 also caused very high cell viability (89.9%). Furthermore, high cell proliferation was seen at early stage for 50Ti, while the cells exposed to 75Ti proliferated more at late stages. Cell differentiation was approximately equal between different groups, and increased by time. Matrix mineralization was higher on the composite surfaces rather than on 0Ti and 100Ti. Moreover, the cells adhered differently to the surfaces of different biomaterials where more spindle-shaped configuration was found on 100Ti, slightly enlarged cells with dendritic shape and early pseudopodia were observed on 75Ti, and more enlarged cells with long dendritic extensions were found on 0Ti, 25Ti, and 50Ti. The results of EDS analysis showed that both Ca and P deposited on the surfaces of all materials, after 20 days of immersion in SBF. Conclusion: Our in-vitro findings demonstrated that the 75Ti, 50Ti, and 25Ti composites have high potential to be used as load-bearing orthopedic materials.
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