番茄离体花药培养方案及再生植株特性

ANNALS OF THE UNIVERSITY OF CRAIOVA, Biology, Horticulture, Food products processing technology, Environmental engineering Pub Date : 2022-12-16 DOI:10.52846/bihpt.v27i63.50

C. Popescu, A. Manolescu, D. Sumedrea, A. Popescu

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引用次数: 0

摘要

本文介绍了一种从花药培养中获得番茄整株植株的方法及其特性。第一年:花药培养的开始和新再生结构的体外发育。第二年:离体驯化植株的表型特征及其SSR标记的分子分析。第3年:前一年结实植株种子萌发获得植株形态特征及SSR标记分子分析。表型描述符和SSR标记显示再生品种与供体品种之间存在差异。从雄激素植物种子中获得的植株的SSR标记遗传谱具有一致性，但与花药供体植株相比存在一些差异，这表明一些再生植株可能具有小孢子起源

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PROTOCOL FOR IN VITRO ANTHER CULTURE IN TOMATO AND THE CHARACTERIZATION OF REGENERATED PLANTS

This paper presents a methodology of obtaining whole tomato plants from anther culture and their characterization. Year 1: the initiation of anther cultures and in vitro development of the new regenerated structures. Year 2: phenotypic characterization of plantlets acclimatized to ex vitro conditions, and their molecular analysis with SSR markers. Year 3: morphological characterization of plants obtained by germination of seeds from plants that fruited in the previous year and their molecular analysis with SSR markers. The phenotype descriptors and the SSR markers revealed differences among the regenerants and the donor variety. The uniformity of the genetic profiles with SSR markers in plants obtained from seeds of androgenic plants, but with several differences in comparison to anther-donor plants, indicates that some of the regenerated plantlets may have microspore origin

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ANNALS OF THE UNIVERSITY OF CRAIOVA, Biology, Horticulture, Food products processing technology, Environmental engineering

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