纳米牛顿介电泳力在原子层沉积氧化物微流体样品制备和蛋白质组学中的应用

S. Emaminejae, M. Javanmare, C. Gupta, R. Dutton, R. W. Davis, R. Howe
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引用次数: 2

摘要

通过增加负电介质电泳(DEP)力的强度,我们展示了一个显着改进的电动驱动和过滤微系统。当施加高交流电压(>20 Vpp)时,采用原子层沉积法沉积无针孔的纳米级氧化薄膜,作为保护电极免受腐蚀的保护层。通过应用高频交流电压信号,电极与电解质缓冲液电容耦合,从而避免了由于绝缘氧化层的存在而导致的电场退化和随后的介电电泳力降低。在这项工作中,我们展示了在两个微流控应用中使用这种深度增强型装置。首先,我们展示了一个芯片平台,用于血液中细胞和高丰度血清蛋白的消耗,这是测定低丰度蛋白质生物标志物的先决条件。对于第二次应用,我们展示了在应用增强的负DEP力后,抗IgG和IgG结合珠100%脱离表面(其强度与典型抗体-抗原相互作用的强度相同)。这种能力提供了针对多种抗原靶标进行基于头的多路检测的可能性,其中在单个微流控通道中,不同的区域用不同的抗体固定,每个抗体靶向不同的抗原。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Applications of nanoNewton dielectrophoretic forces using atomic layer deposited oxides for microfluidic sample preparation and proteomics
By increasing the strength of the negative dielectrophoresis (DEP) force, we demonstrate a significantly improved electrokinetic actuation and filtering microsystem. A pinhole-free, nanometer-scale, thin film oxide was deposited using atomic layer deposition, as a protective layer to protect the electrodes from corrosion, when applying high AC voltages (>20 Vpp) at the electrodes. The electrodes were capacitively coupled to the electrolyte buffer by the application of a high frequency AC voltage signal, thus avoiding electric field degradation and the consequent reduction in dielectrophoresis force due to the presence of the insulating oxide layer. In this work, we demonstrated the use of this DEP-enhanced device for two microfluidic applications. First, we demonstrate an on-chip platform for the depletion of cells and highly abundant serum proteins in blood, which is a prerequisite to assay low-abundance protein biomarkers. For the second application, we show 100% detachment of anti-IgG and IgG bound beads (which is on the same order of magnitude in strength as typical antibody-antigen interactions) from the surface, upon the application of the enhanced negative DEP force. This capability offers the possibility of performing a bead-based multiplexed assay against multiple antigen targets where in a single microfluidic channel various regions are immobilized with a different antibody, each targeting a different antigen.
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