棕榈糖浆中加入干姜、黑胡椒和长胡椒的核磁共振植物化学分析

Ravi Shankar Lankalapalli, Ambili Sasikumar Athira, Ramkumar Kiruthika, Nagaraja Ingaladal, K. Krishnakumar, Natakkakath Kaliyathan Raveena, Biju Gopika, Mullan Velandy Reshma
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引用次数: 0

摘要

Trikatu是许多印度阿育吠陀药物的重要成分,是三种香料的混合物,即干姜,黑胡椒和长胡椒,以其特有的辛辣而闻名。为了将特里卡图转化为一种被广泛接受的美味形式,我们将这三种香料混合成煎剂形式,并将它们添加到从棕榈尼拉制备的糖浆中,这就是“特里卡图糖浆”(TS)。最近,我们报道了TS[1]的体内免疫调节特性。香料的免疫调节作用主要归因于某些植物化学物质的存在。鉴于香料中植物化学物质作为免疫调节剂的重要性,有必要对香料配方中的这些生物活性物质进行彻底的研究。在目前的研究中,我们专注于了解香料和糖浆基植物化学物质在配方产品中的保留情况,这有助于ts的产品标准化。与HPLC或质谱工具相比,核磁共振是一种高度可靠的工具,可以明确确认植物化学物质的结构。植物化学物质的核磁共振光谱,无论是纯形式还是当植物化学物质是混合物的一部分时,都可以对食品基质有机提取物中的植物化学物质混合物进行定性和定量研究。因此,本文描述了从TS有机提取物中分离的化合物的NMR光谱比较。使用diion®HP-20对TS进行分选,根据极性对化合物进行分选。丙酮部分的柱层析纯化有助于化合物1 (pellitorine)、化合物2(胡椒碱)、化合物3-5 (trienamides)和化合物6 (pipataline)的有效分离。乙腈馏分得到化合物7(尿苷)和8 (3- o-甲基肌醇),这两种化合物在三种香料和棕榈中均未见报道。TS的丙酮部分及其植物化学物质1-6的定性显示作为TS的指纹图谱。总之,TS是棕榈糖浆中具有免疫调节潜力1的美味香料营养保健品,我们对其有机部分的植物化学成分进行了彻底的研究。使用Diaion®HP-20对TS进行分离,随后进行闪蒸纯化和柱层析,有助于分离出重要的植物化学物质。我们报告了核磁共振作为一种可靠和有效的工具,用于指纹识别配方,营养药品等植物化学物质,这有助于确定其真实性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
NMR-based phytochemical profiling of palmyra palm syrup infused with dry ginger, black pepper, and long pepper
Trikatu, a vital ingredient in many Indian Ayurvedic drugs, is a consortium of three spices, viz. dry ginger, black pepper, and long pepper, known for its peculiar pungency. To convert Trikatu into a widely acceptable palatable form, we blended these three spices in a decoction form and added them to syrup prepared from palmyra palm neera, which resulted in ‘Trikatu Syrup’ (TS). Recently, we reported in vivo immunomodulatory properties of TS [1]. The immunomodulatory effects of spices are attributed largely to the presence of certain phytochemicals. The importance of phytochemicals in spices as immunomodulatory agents necessitate a thorough investigation of these bioactives in formulations comprising spices. In the present study, we have focused on understanding the retention of spice and syrup-based phytochemicals in the formulated product that assists in product standardization of TS. NMR serves as a highly reliable tool for explicit structural confirmation of phytochemicals when compared to HPLC or mass spectrometry tools. NMR spectra of a phytochemical, whether in pure form or when the phytochemical is a part of the mixture, enable qualitative and quantitative studies with a mixture of phytochemicals in organic extracts of food matrices. Hence, the NMR spectral comparison of compounds isolated from the organic extracts of TS is described here. Fractionation of TS using Diaion® HP-20 resulted in the partitioning of compounds based on their polarity. Purification of the acetone fraction by column chromatography aided in the efficient isolation of compound 1 (pellitorine), compound 2 (piperine), compounds 3-5 (trienamides), and compound 6 (pipataline). Acetonitrile fraction yielded compound 7 (uridine) and compound 8 (3-O-methyl-myo-inositol), which were neither reported in the three spices nor palmyra palm. A qualitative display of the acetone fraction of TS with its phytochemicals 1-6 served as a fingerprint of TS. In summary, TS, a palatable spice-based nutraceutical in palmyra palm syrup with immunomodulatory potential 1, was thoroughly investigated for the phytochemical composition of its organic fractions. The process of fractionating TS using Diaion® HP-20, subsequent flash purification, and column chromatography facilitated the isolation of prominent phytochemicals. We report the utility of NMR as a reliable and efficient tool for fingerprinting phytochemicals in formulations, nutraceuticals, etc., which assists in ascertaining their authenticity.
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