Synthesis of rare Earth fluorescent probe intermediates by homogeneous time-resolved fluorescence immunoassay

In order to prepare a novel cave-like bifunctional chelating agent for homogeneous phase time-resolved fluorescence immunoassay, using 2-benzyl oxo carbonyl 6-tert-butyl oxo carbonyl. The intermediate, 5-amino-(2-aminoethyl)-H(2,2)- aminotert-butyl ester E-14, was synthesized by five steps of reduction, oxidation, amination and hydrogenation of -L-lysine E-7. The synthesis methods and conditions of the first steps were mainly studied, and the molecular structure was analyzed by means of Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared Spectrometer (FTIR), Mass Spectrometry (MS). It provides a valuable intermediate and preparation method for the application of homogeneous time resolved fluorescence immunoassay.