持续高同型血条件下成年大鼠心脏的亚显微变化

R. Kaminsky, I. Dzevulska, A. Yanchyshyn, R. Matkivska, I. Samborska
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引用次数: 1

摘要

心血管疾病是全世界死亡和残疾的主要原因。已经确定,近年来患有这种病理的患者数量显著增加,迫使研究人员、科学家和医生寻找心血管疾病的危险因素,其中之一是高同型半胱氨酸血症(HHCys)。本研究的目的是研究成年大鼠心脏在HHCys条件下的亚显微变化特征。实验研究采用22只成年(6-8个月)雄性非线性白色大鼠(斯特拉斯堡,1986;基辅,2001)。在实验中,动物被分为两组:对照组和实验组。通过给实验组大鼠灌胃硫代内酯同型半胱氨酸(HCys) 200 mg/kg体重,连续60天模拟持久性HHCys。在PEM - 125K电镜下对超薄切片进行了研究。研究发现,给成年大鼠注射200 mg/kg剂量的硫代内酯HCys可导致动物心脏出现营养不良和破坏性变化。心内膜结缔组织明显水肿,心肌微循环道成分紊乱。毛细血管内皮细胞可见局部启蒙、胞浆水肿和局部异染色质凝聚。心肌细胞肌原纤维增厚,线粒体肿胀,嵴部分破坏,光滑内质网小管和t小管扩张。这些结果表明,在成年大鼠中,HHCys引起了实验动物心内膜、心肌和微循环道的病理改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Submicroscopic changes in the heart of adult rats under conditions of persistent hyperhomocystemia
Cardiovascular diseases are the leading cause of death and disability worldwide. It has been established that in recent years there has been a significant increase in the number of patients with this pathology, forcing researchers, scientists and physicians to look for risk factors of cardiovascular diseases, one of which is hyperhomocysteinemia (HHCys). The aim of the research is to study the features of submicroscopic changes in the heart of adult rats under conditions of HHCys. Experimental studies were performed on 22 white nonlinear adult (6-8 months) male rats in accordance with the principles of bioethics (Strasbourg, 1986; Kyiv, 2001). During the experiment, the animals were divided into two groups – control and experimental. Simulation of persistent HHCys was achieved by administering to rats the experimental group thiolactone homocysteine (HCys) at a dose of 200 mg/kg body weight intragastrally for 60 days. Ultrathin sections were studied in the PEM – 125K electron microscope. It was found that the introduction of thiolactone HCys to adult rats at a dose of 200 mg/kg causes the development of dystrophic and destructive changes in the heart of animals. Significant connective tissue edema was observed in the endocardium, and disturbances in the components of the microcirculatory tract were detected in the myocardium. Local enlightenment, cytoplasmic edema and local condensation of heterochromatin in hypertrophied nuclei were detected in hemocapillary endothelial cells. In cardiomyocytes, myofibrils are thickened, mitochondria are swollen with partial destruction of the cristae, tubules of smooth endoplasmic reticulum and T-tubules are dilated. These findings indicate that in adult rats HHCys caused the development of pathological changes in the endocardium, myocardium of experimental animals and in the microcirculatory tract.
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