白血病患者白细胞介素-4内含子3 VNTR多态性基因

A. Ahmed, R. Abdelgadir, A. M. Muddathir, E. Elshibli, imad Mohamed Fadl Elmula
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引用次数: 3

摘要

背景:白血病是一组白细胞及其前体的慢性恶性疾病。白细胞介素-4 (IL-4)是炎症细胞因子,决定b细胞、肥大细胞、红细胞祖细胞的活化和分化。一些研究调查了IL-4内含子3可变数目串联重复序列(VNTR)多态性与人类癌症风险之间的关系;然而,这种关联并未在白血病患者中进行研究。材料与方法:本研究旨在探讨白血病患者IL-4基因内含子3(VNTR)多态性的基因型和等位基因频率。这项研究包括231名白血病患者和163名健康对照者。采用改良盐析法从3 ml抗凝静脉血中分离基因组DNA。特异引物PCR检测IL-4内含子3 VNTR多态性。数据采用SPSS 21版软件进行分析。使用P值、优势比(OR)和相应的95%置信区间(CI)来估计相关性的强度。结果:与所有对照组相比,25.9%(60/231)的白血病患者存在等位基因频率,74.1%(171/231)的白血病患者存在等位基因频率,P值为0.00,白血病危险因子为4.617倍。白血病患者内含子3 VNTR多态性P1P1、P2P2、P1P2基因型频率与对照组差异有统计学意义(P值=0.00)。结果显示,P1P1和P1P2等位基因发生白血病的风险高于P2P2 (OR: P1P1 1.24, 95% CI: 0.675-2.279;Or p1p2: 1.24, 95% ci: 0.568-2.7;或p2p2:0.72, 95% ci: 0.398-1.3)。结论:IL-4内含子3 VNTR多态性可能影响白血病的发生;这可以作为疾病过程中的早期预后指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interleukin-4 Intron 3 VNTR Polymorphism Gene in Leukemic Patients
Background: Leukemia is a group of chronic malignant disorders of White blood cells and its precursor. Interleukin-4 (IL-4) is inflammatory cytokine that determines the activation and differentiation of B-cells, mast cells, erythroid progenitors. Several studies have investigated the association between IL-4 intron 3 variable number of tandem repeats (VNTR) polymorphism and cancer risk in humans; however, this association is not investigated among patients with leukemia. Material and Methods: The present study aimed to investigate genotype and allele frequencies of IL-4 gene intron 3(VNTR) polymorphism in patients with leukemia compared to healthy control. The study included 231 patients with leukemia and 163 healthy controls. Genomic DNA was isolated from 3 ml of anticoagulated venous blood samples by modified salting out method. IL-4 intron 3 VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers. The data were analysed using SPSS software program version 21. P value, Odds ratio (OR) and corresponding 95% confidence interval (CI) were used to estimate the strength of the association. Results: The allele frequency was showed in 25.9% (60/231) leukemic patients while 74.1% (171/231) showed absence of allele compared with the presence of allele in all control group with significance differences of P value=0.00 and risk factor of 4.617 times for leukemia. The frequencies of P1P1, P2P2, and P1P2 genotypes of intron 3 VNTR polymorphism in leukemic patients were significantly different from control group P value=0.00.The result showed, P1P1 and P1P2 allele were highly risk for developing leukemia than P2P2 (OR: P1P1 1.24, 95% CI: 0.675-2.279; OR P1P2: 1.24, 95% CI: 0.568-2.7; OR P2P2:0.72, 95% CI: 0.398-1.3). Conclusion: IL-4 intron 3 VNTR polymorphism could influences in the risk of leukemia; this could be used as early prognostic marker in the course of the disease.
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