A. Ahmed, R. Abdelgadir, A. M. Muddathir, E. Elshibli, imad Mohamed Fadl Elmula
{"title":"白血病患者白细胞介素-4内含子3 VNTR多态性基因","authors":"A. Ahmed, R. Abdelgadir, A. M. Muddathir, E. Elshibli, imad Mohamed Fadl Elmula","doi":"10.4172/2155-9864.1000357","DOIUrl":null,"url":null,"abstract":"Background: Leukemia is a group of chronic malignant disorders of White blood cells and its precursor. Interleukin-4 (IL-4) is inflammatory cytokine that determines the activation and differentiation of B-cells, mast cells, erythroid progenitors. Several studies have investigated the association between IL-4 intron 3 variable number of tandem repeats (VNTR) polymorphism and cancer risk in humans; however, this association is not investigated among patients with leukemia. \nMaterial and Methods: The present study aimed to investigate genotype and allele frequencies of IL-4 gene intron 3(VNTR) polymorphism in patients with leukemia compared to healthy control. The study included 231 patients with leukemia and 163 healthy controls. Genomic DNA was isolated from 3 ml of anticoagulated venous blood samples by modified salting out method. IL-4 intron 3 VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers. The data were analysed using SPSS software program version 21. P value, Odds ratio (OR) and corresponding 95% confidence interval (CI) were used to estimate the strength of the association. \nResults: The allele frequency was showed in 25.9% (60/231) leukemic patients while 74.1% (171/231) showed absence of allele compared with the presence of allele in all control group with significance differences of P value=0.00 and risk factor of 4.617 times for leukemia. The frequencies of P1P1, P2P2, and P1P2 genotypes of intron 3 VNTR polymorphism in leukemic patients were significantly different from control group P value=0.00.The result showed, P1P1 and P1P2 allele were highly risk for developing leukemia than P2P2 (OR: P1P1 1.24, 95% CI: 0.675-2.279; OR P1P2: 1.24, 95% CI: 0.568-2.7; OR P2P2:0.72, 95% CI: 0.398-1.3). \nConclusion: IL-4 intron 3 VNTR polymorphism could influences in the risk of leukemia; this could be used as early prognostic marker in the course of the disease.","PeriodicalId":182392,"journal":{"name":"Journal of Blood Disorders and Transfusion","volume":"353 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2016-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Interleukin-4 Intron 3 VNTR Polymorphism Gene in Leukemic Patients\",\"authors\":\"A. Ahmed, R. Abdelgadir, A. M. Muddathir, E. Elshibli, imad Mohamed Fadl Elmula\",\"doi\":\"10.4172/2155-9864.1000357\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Leukemia is a group of chronic malignant disorders of White blood cells and its precursor. Interleukin-4 (IL-4) is inflammatory cytokine that determines the activation and differentiation of B-cells, mast cells, erythroid progenitors. Several studies have investigated the association between IL-4 intron 3 variable number of tandem repeats (VNTR) polymorphism and cancer risk in humans; however, this association is not investigated among patients with leukemia. \\nMaterial and Methods: The present study aimed to investigate genotype and allele frequencies of IL-4 gene intron 3(VNTR) polymorphism in patients with leukemia compared to healthy control. The study included 231 patients with leukemia and 163 healthy controls. Genomic DNA was isolated from 3 ml of anticoagulated venous blood samples by modified salting out method. IL-4 intron 3 VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers. The data were analysed using SPSS software program version 21. P value, Odds ratio (OR) and corresponding 95% confidence interval (CI) were used to estimate the strength of the association. \\nResults: The allele frequency was showed in 25.9% (60/231) leukemic patients while 74.1% (171/231) showed absence of allele compared with the presence of allele in all control group with significance differences of P value=0.00 and risk factor of 4.617 times for leukemia. The frequencies of P1P1, P2P2, and P1P2 genotypes of intron 3 VNTR polymorphism in leukemic patients were significantly different from control group P value=0.00.The result showed, P1P1 and P1P2 allele were highly risk for developing leukemia than P2P2 (OR: P1P1 1.24, 95% CI: 0.675-2.279; OR P1P2: 1.24, 95% CI: 0.568-2.7; OR P2P2:0.72, 95% CI: 0.398-1.3). \\nConclusion: IL-4 intron 3 VNTR polymorphism could influences in the risk of leukemia; this could be used as early prognostic marker in the course of the disease.\",\"PeriodicalId\":182392,\"journal\":{\"name\":\"Journal of Blood Disorders and Transfusion\",\"volume\":\"353 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-05-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Blood Disorders and Transfusion\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2155-9864.1000357\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Blood Disorders and Transfusion","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2155-9864.1000357","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Interleukin-4 Intron 3 VNTR Polymorphism Gene in Leukemic Patients
Background: Leukemia is a group of chronic malignant disorders of White blood cells and its precursor. Interleukin-4 (IL-4) is inflammatory cytokine that determines the activation and differentiation of B-cells, mast cells, erythroid progenitors. Several studies have investigated the association between IL-4 intron 3 variable number of tandem repeats (VNTR) polymorphism and cancer risk in humans; however, this association is not investigated among patients with leukemia.
Material and Methods: The present study aimed to investigate genotype and allele frequencies of IL-4 gene intron 3(VNTR) polymorphism in patients with leukemia compared to healthy control. The study included 231 patients with leukemia and 163 healthy controls. Genomic DNA was isolated from 3 ml of anticoagulated venous blood samples by modified salting out method. IL-4 intron 3 VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers. The data were analysed using SPSS software program version 21. P value, Odds ratio (OR) and corresponding 95% confidence interval (CI) were used to estimate the strength of the association.
Results: The allele frequency was showed in 25.9% (60/231) leukemic patients while 74.1% (171/231) showed absence of allele compared with the presence of allele in all control group with significance differences of P value=0.00 and risk factor of 4.617 times for leukemia. The frequencies of P1P1, P2P2, and P1P2 genotypes of intron 3 VNTR polymorphism in leukemic patients were significantly different from control group P value=0.00.The result showed, P1P1 and P1P2 allele were highly risk for developing leukemia than P2P2 (OR: P1P1 1.24, 95% CI: 0.675-2.279; OR P1P2: 1.24, 95% CI: 0.568-2.7; OR P2P2:0.72, 95% CI: 0.398-1.3).
Conclusion: IL-4 intron 3 VNTR polymorphism could influences in the risk of leukemia; this could be used as early prognostic marker in the course of the disease.