嘧啶和cavinton衍生物对大鼠脑局灶性缺血条件下生物电势变化的影响

{"title":"嘧啶和cavinton衍生物对大鼠脑局灶性缺血条件下生物电势变化的影响","authors":"","doi":"10.47026/2413-4864-2023-1-101-107","DOIUrl":null,"url":null,"abstract":"Acute disorders of cerebral hemodynamics lead to the development of socially and demographically significant diseases, as a result of which they are one of the main health problems requiring a rational pharmacological approach. In the pathogenesis of pathologies of cerebral circulation, in addition to a decrease in blood flow, an important role is played by a violation of the bioelectric activity of brain tissues, which is evidenced by changes in the frequency and amplitude of oscillations on the encephalogram. Pyrimidine derivatives have proven themselves as potential cerebroprotectors, as a result of which we considered them as means capable of correcting electroencephalogram disorders in ischemic brain tissues. In this regard, the purpose of this work was to study the effect of pyrimidine and cavinton derivatives on the change in bioelectric potential under conditions of focal central ischemia in rats. Materials and methods. The study was conducted on 40 male rats of the Wistar line (m = 220–220 g). Focal cerebral ischemia of rats was reproduced by occlusion of the left middle cerebral artery. The animals were divided into 4 equal groups, all groups, except the first, were simulated pathology under chloral hydrate anesthesia (350 mg/kg). The first group – falsely operated rats, the second – individuals of negative control. The reference drug cavinton (3.2 mg/kg) and the pi-rimidine derivative PIR-10 (50 mg/kg) were administered to the third and fourth groups within 3 days after surgery. The electrical activity of the rat brain (delta-, theta-, alpha-, high-frequency beta-range in the frontal and parietal lobes of the left hemisphere) was evaluated after 3 days by electroencephalography (EEG) using the Neuron-Spectrum 1 encephalograph (Neurosoft, Russia). Statistical processing was carried out using the STATISTICA 8.0 application software package (StatSoft, Inc., USA). Results of the study. In the rats of the negative control group, there was a marked deterioration in bioelectric potential, which was noted in an increase in theta and delta rhythms and a decrease in alpha and high-frequency beta rhythms. The introduction of the compound PIR-10 to animals contributed to a decrease in delta rhythm by 39,8% (p < 0,05) (FP1-A1) and 56,3% (p < 0,05) (C3-A1), theta rhythm – by 23,9% (p < 0,05) (FP1-A1) and 39,4% (p < 0,05) (C3-A1), the amplitude of the alpha rhythm increased by 75,3% (p < 0,05) (C3-A1), the high-frequency beta rhythm by 25,9% (p<0,05) (FP1-A1) and 41,4% (p < 0,05) (C3-A1). Findings. The experimental derivative of pyrimidine PIR-10, equally with cavinton, contributed to the restoration of bioelectric rhythm in the form of a decrease in the amplitude of delta and theta rhythms and an increase in the amplitude of alpha and high-frequency beta rhythms in the frontal and parietal regions of the left hemisphere of the rat brain.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"51 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"THE EFFECT OF PYRIMIDINE AND CAVINTON DERIVATIVES ON THE CHANGE BIOELECTRIC POTENTIAL IN THE CONDITIONS OF FOCAL CEREBRAL ISCHEMIA OF THE RAT BRAIN\",\"authors\":\"\",\"doi\":\"10.47026/2413-4864-2023-1-101-107\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Acute disorders of cerebral hemodynamics lead to the development of socially and demographically significant diseases, as a result of which they are one of the main health problems requiring a rational pharmacological approach. In the pathogenesis of pathologies of cerebral circulation, in addition to a decrease in blood flow, an important role is played by a violation of the bioelectric activity of brain tissues, which is evidenced by changes in the frequency and amplitude of oscillations on the encephalogram. Pyrimidine derivatives have proven themselves as potential cerebroprotectors, as a result of which we considered them as means capable of correcting electroencephalogram disorders in ischemic brain tissues. In this regard, the purpose of this work was to study the effect of pyrimidine and cavinton derivatives on the change in bioelectric potential under conditions of focal central ischemia in rats. Materials and methods. The study was conducted on 40 male rats of the Wistar line (m = 220–220 g). Focal cerebral ischemia of rats was reproduced by occlusion of the left middle cerebral artery. The animals were divided into 4 equal groups, all groups, except the first, were simulated pathology under chloral hydrate anesthesia (350 mg/kg). The first group – falsely operated rats, the second – individuals of negative control. The reference drug cavinton (3.2 mg/kg) and the pi-rimidine derivative PIR-10 (50 mg/kg) were administered to the third and fourth groups within 3 days after surgery. The electrical activity of the rat brain (delta-, theta-, alpha-, high-frequency beta-range in the frontal and parietal lobes of the left hemisphere) was evaluated after 3 days by electroencephalography (EEG) using the Neuron-Spectrum 1 encephalograph (Neurosoft, Russia). Statistical processing was carried out using the STATISTICA 8.0 application software package (StatSoft, Inc., USA). Results of the study. In the rats of the negative control group, there was a marked deterioration in bioelectric potential, which was noted in an increase in theta and delta rhythms and a decrease in alpha and high-frequency beta rhythms. The introduction of the compound PIR-10 to animals contributed to a decrease in delta rhythm by 39,8% (p < 0,05) (FP1-A1) and 56,3% (p < 0,05) (C3-A1), theta rhythm – by 23,9% (p < 0,05) (FP1-A1) and 39,4% (p < 0,05) (C3-A1), the amplitude of the alpha rhythm increased by 75,3% (p < 0,05) (C3-A1), the high-frequency beta rhythm by 25,9% (p<0,05) (FP1-A1) and 41,4% (p < 0,05) (C3-A1). Findings. The experimental derivative of pyrimidine PIR-10, equally with cavinton, contributed to the restoration of bioelectric rhythm in the form of a decrease in the amplitude of delta and theta rhythms and an increase in the amplitude of alpha and high-frequency beta rhythms in the frontal and parietal regions of the left hemisphere of the rat brain.\",\"PeriodicalId\":249759,\"journal\":{\"name\":\"Acta medica Eurasica\",\"volume\":\"51 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta medica Eurasica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47026/2413-4864-2023-1-101-107\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta medica Eurasica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47026/2413-4864-2023-1-101-107","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

急性脑血流动力学紊乱导致社会和人口统计学上重大疾病的发展,因此它们是需要合理药理学方法的主要健康问题之一。在脑循环病理的发病机制中,除了血流量减少外,脑组织生物电活动的破坏也起着重要作用,脑电图上振荡的频率和幅度的变化证明了这一点。嘧啶衍生物已被证明是潜在的脑保护剂,因此我们认为它们是能够纠正缺血性脑组织脑电图障碍的手段。因此,本研究的目的是研究嘧啶和cavinton衍生物对大鼠局灶性中枢缺血条件下生物电位变化的影响。材料和方法。以Wistar系雄性大鼠40只(m = 220 ~ 220 g)为实验对象,通过阻断左大脑中动脉复制大鼠局灶性脑缺血。将大鼠分为4组,除第1组外,其余各组在水合氯醛(350 mg/kg)麻醉下模拟病理。第一组为假手术大鼠,第二组为阴性对照个体。第三组和第四组在术后3 d内给予参比药物cavinton (3.2 mg/kg)和pi-嘧啶衍生物PIR-10 (50 mg/kg)。3天后,使用神经元-频谱1脑电图仪(Neurosoft,俄罗斯)通过脑电图(EEG)评估大鼠大脑的电活动(左半球额叶和顶叶的δ, θ, α,高频β范围)。统计处理采用STATISTICA 8.0应用软件包(StatSoft, Inc., USA)。研究结果。在阴性对照组的大鼠中,生物电电位明显恶化,表现为θ和δ节律增加,α和高频β节律减少。化合物PIR-10的引入使动物的δ节律降低了39.8% (p< 0.05) (FP1-A1)和56.3% (p< 0.05) (C3-A1), θ节律降低了23.9% (p< 0.05) (FP1-A1)和39.4% (p< 0.05) (C3-A1), α节律的振幅增加了75.3% (p< 0.05) (C3-A1),高频β节律增加了25.9% (p< 0.05) (FP1-A1)和41.4% (p< 0.05) (C3-A1)。发现。与cavinton一样,实验中嘧啶衍生物PIR-10对大鼠左脑额叶和顶叶区域的δ和θ节律幅度降低,α和高频β节律幅度增加等生物电节律的恢复也有贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
THE EFFECT OF PYRIMIDINE AND CAVINTON DERIVATIVES ON THE CHANGE BIOELECTRIC POTENTIAL IN THE CONDITIONS OF FOCAL CEREBRAL ISCHEMIA OF THE RAT BRAIN
Acute disorders of cerebral hemodynamics lead to the development of socially and demographically significant diseases, as a result of which they are one of the main health problems requiring a rational pharmacological approach. In the pathogenesis of pathologies of cerebral circulation, in addition to a decrease in blood flow, an important role is played by a violation of the bioelectric activity of brain tissues, which is evidenced by changes in the frequency and amplitude of oscillations on the encephalogram. Pyrimidine derivatives have proven themselves as potential cerebroprotectors, as a result of which we considered them as means capable of correcting electroencephalogram disorders in ischemic brain tissues. In this regard, the purpose of this work was to study the effect of pyrimidine and cavinton derivatives on the change in bioelectric potential under conditions of focal central ischemia in rats. Materials and methods. The study was conducted on 40 male rats of the Wistar line (m = 220–220 g). Focal cerebral ischemia of rats was reproduced by occlusion of the left middle cerebral artery. The animals were divided into 4 equal groups, all groups, except the first, were simulated pathology under chloral hydrate anesthesia (350 mg/kg). The first group – falsely operated rats, the second – individuals of negative control. The reference drug cavinton (3.2 mg/kg) and the pi-rimidine derivative PIR-10 (50 mg/kg) were administered to the third and fourth groups within 3 days after surgery. The electrical activity of the rat brain (delta-, theta-, alpha-, high-frequency beta-range in the frontal and parietal lobes of the left hemisphere) was evaluated after 3 days by electroencephalography (EEG) using the Neuron-Spectrum 1 encephalograph (Neurosoft, Russia). Statistical processing was carried out using the STATISTICA 8.0 application software package (StatSoft, Inc., USA). Results of the study. In the rats of the negative control group, there was a marked deterioration in bioelectric potential, which was noted in an increase in theta and delta rhythms and a decrease in alpha and high-frequency beta rhythms. The introduction of the compound PIR-10 to animals contributed to a decrease in delta rhythm by 39,8% (p < 0,05) (FP1-A1) and 56,3% (p < 0,05) (C3-A1), theta rhythm – by 23,9% (p < 0,05) (FP1-A1) and 39,4% (p < 0,05) (C3-A1), the amplitude of the alpha rhythm increased by 75,3% (p < 0,05) (C3-A1), the high-frequency beta rhythm by 25,9% (p<0,05) (FP1-A1) and 41,4% (p < 0,05) (C3-A1). Findings. The experimental derivative of pyrimidine PIR-10, equally with cavinton, contributed to the restoration of bioelectric rhythm in the form of a decrease in the amplitude of delta and theta rhythms and an increase in the amplitude of alpha and high-frequency beta rhythms in the frontal and parietal regions of the left hemisphere of the rat brain.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信