消毒剂“Enzidez”的杀菌性能

V. Kozhyn
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引用次数: 0

摘要

消毒作为兽医和卫生措施的一个组成部分,可确保牲畜的健康,提高动物生产力并获得安全的原料。因此,开发具有广泛抗菌作用的同时又能影响细菌生物膜的消毒剂具有重要意义。研究了新型消毒液“Enzidez”的最低杀菌浓度、酚类系数和蛋白质指数。实验采用大肠杆菌(055K59№3912 / 41)、金黄色葡萄球菌(ATCC 25923)和铜绿假单胞菌(27/99)的博物馆试验菌株。采用普遍采用的悬浮液法测定了Enzidez消毒液的最低杀菌浓度、酚比和蛋白质指数。结果发现,与金黄色葡萄球菌相比,大肠杆菌和铜绿假单胞菌对“Enzidez”消毒剂更敏感。暴露15 min后,金黄色葡萄球菌的最低杀菌稀释度平均是大肠杆菌的1.9倍(p <0.05),铜绿假单胞菌的最低杀菌稀释度平均是3.8倍(p <0.05)。暴露30min时,金黄色葡萄球菌与大肠杆菌和铜绿假单胞菌的最小杀菌稀释度差异分别为1.3倍和2.7倍(p <0.05)。因此,随着暴露时间从15分钟增加到30分钟,用于杀灭革兰氏阳性和革兰氏阴性微生物的药剂浓度显著降低。结果表明,“Enzidez”消毒液对金黄色葡萄球菌(S. aureus№ATCC 25923)的杀菌活性平均比苯酚溶液强12.7倍。对大肠杆菌和铜绿假单胞菌的抑菌活性分别比苯酚强24.0倍和28.8倍。培养基中蛋白质(血清)的存在导致Enzides溶液的杀菌活性略有下降。特别是,如果培养基中含有10%的血清,则Enzides对金黄色葡萄球菌的杀菌活性平均降低1.4倍。蛋白存在时,酶联蛋白对大肠杆菌和铜绿假单胞菌的抑菌活性分别下降1.35倍和1.45倍。因此,即使在培养基中存在蛋白质的情况下,消毒剂“Enzidez”也具有相当的活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bactericidal properties of disinfectant «Enzidez»
Disinfection as a component of veterinary and sanitary measures ensures the well-being of livestock, increasing of animal productivity and obtaining safe raw materials. Therefore, the development of disinfectants that have a wide range of antimicrobial action and at the same time affect bacterial biofilms is relevant. The aim of the study was to determine the minimum bactericidal concentration, phenolic coefficient and protein index of the new disinfectant "Enzidez". Museum strains of test cultures of E. coli (055K59 №3912 / 41), S. aureus (ATCC 25923) and P. aeruginosa (27/99) were used in the experiments. The minimum bactericidal concentration of Enzidez disinfectant solutions, the phenolic ratio and the protein index were determined by generally accepted suspension methods. It was found that the microorganisms E. coli and P. aeruginosa were more sensitive to the disinfectant "Enzidez" compared to S. aureus. By exposure for 15 min the minimum bactericidal dilution against S. aureus was on average 1.9 times (p <0.05) higher than the bactericidal dilution against E. coli and 3.8 times (p <0.05) in relation to P. aeruginosa. By 30-minute exposure the difference between the minimum bactericidal dilution against S. aureus and against E. coli and P. aeruginosa was 1.3 and 2.7 times less (p <0.05), respectively. Thus, with increasing exposure time from 15 to 30 minutes the concentration of the agent for the destruction of gram-positive and gram-negative microorganisms decreases significantly. It was found that the bactericidal activity of the disinfectant "Enzidez" against the strain S. aureus №ATCC 25923 was on average 12.7 times stronger than the bactericidal action of the phenol solution. The bactericidal activity of Enzides against E. coli and P. aeruginosa strains was 24.0 and 28.8 times stronger, respectively compared with the action of phenol. The presence of protein (blood serum) in the medium leads to a slight decrease in the bactericidal activity of Enzides solutions. In particular, the bactericidal activity of Enzides against S. aureus is reduced by an average of 1.4 times, provided the presence of 10% of serum in the medium. The bactericidal activity of Enzides against E. coli and P. aeruginosa in the presence of protein decreased by 1.35 and 1.45 times, respectively. Thus, the disinfectant "Enzidez" is quite active even in the presence of protein in the medium.
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