乌克兰黑白奶牛种群csn2和csn3位点单倍型频率分布分析

R. Kulibaba, M. Sakhatskyi, Yurii Liashenko
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引用次数: 1

摘要

对乌克兰黑白奶牛种群的β -酪蛋白(CSN2)和kappa-酪蛋白(CSN3)位点进行遗传结构研究,分析单倍型频率分布。CSN2和CSN3分别采用AS-PCR(等位基因特异性PCR)和PCR- rflp(限制性内切分析)检测实验位点的多态性。根据研究结果确定基因型和等位基因频率、观察杂合度(Ho)和期望杂合度(He)值、赖特固定指数(Fis)和有效等位基因数(ne)。单倍型频率通过EH+程序计算em -算法确定。利用2LD程序计算了连杆机构偏离平衡状态的标准化度量。根据研究结果,在乌克兰黑白奶牛种群中发现了两个实验位点的多态性。在CSN2位点,A1等位基因频率为0.44;A2 - 0.56。在CSN3位点,A等位基因频率为0.80;等位基因B - 0.20。实验牛群在两个多态位点上均处于遗传平衡状态。单倍型频率分布分析发现,CSN2A1-CSN3A单倍型频率最高(0.46),CSN2A2-CSN3B单倍型频率最低(0.085)。在乌克兰黑白奶牛品种的实验牛种群中,通过连锁没有偏离平衡状态(D ' = 0.33;CSN2和CSN3基因座等位基因变异之间的差异显著小于临界值。这表明单倍型频率的分布是相应等位基因频率分布特殊性的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ANALYSIS OF HAPLOTYPE FREQUENCY DISTRIBUTION BY CSN2 AND CSN3 LOCUS IN THE CATTLE POPULATION OF THE UKRAINIAN BLACK-AND-WHITE DAIRY BREED
The study of the genetic structure of the cow population of the Ukrainian Black-and-White dairy breed were carried out in loci of beta-casein (CSN2) and kappa-casein (CSN3) loci, and the distribution of haplotype frequencies was analyzed. The polymorphism of the experimental loci was determined using AS-PCR (allele-specific PCR) in the case of CSN2 and PCR-RFLP (restriction analysis) in the case of CSN3. The frequencies of genotypes and alleles, the value of observable (Ho) and expected (He) heterozygosity, the Wright fixation index (Fis) and the effective number of alleles (ne) were determined based on the results of the studies. Haplotype frequencies were determined by calculating the EM-algorithm using the EH+ program. Calculations of the standardized measure of deviation by linkage from the equilibrium state were made using the 2LD program. According to the results of the studies, polymorphism was revealed for both experimental loci in the cattle population of the Ukrainian Black-and-White dairy breed. At the CSN2 locus, the A1 allele frequency was 0.44; A2 – 0.56. At the CSN3 locus, the A allele frequency was 0.80; allele B – 0.20. The experimental cattle population was in a state of genetic equilibrium in accordance with both polymorphic loci. Based on the analysis of the haplotype frequencies distribution, haplotype CSN2A1-CSN3A was found to have the highest frequency (0,46), haplotype CSN2A2-CSN3B had the smallest (0,085). In the experimental cattle population of the Ukrainian Black-and-White dairy breed there is no deviation from the equilibrium state by linkage (D′ = 0.33; which is significantly less than the critical value) between the identified allelic variants of the CSN2 and CSN3 loci. This indicates that the distribution of haplotype frequencies is the result of the particularities of the corresponding alleles frequency distribution.
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