南极动物环境变化的生化标志物:乔治王岛金钟湾潮间带环境监测用小藻能量代谢酶的筛选

C. N. K. Suda, M. Feijó-Oliveira, Edson Rodrigues Junior, G. Vani, L. Donatti, H. P. Lavrado, E. Rodrigues
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引用次数: 0

摘要

南极帽贝居住在金钟湾的潮间带海岸。测定了该物种能量代谢酶的活性,以评价其作为潮间带环境变化的生化标志物的潜力。2011年2月,在金钟湾的六个潮间带地点:乌尔曼点、植物学点、蓬塔广场、亨内昆点、巴西避难所2号和巴西南极站指挥官费拉兹的油罐前收集了5只帽贝。对这些帽贝的鳃和足部组织进行了E酶活性测定。脚部组织中苹果酸脱氢酶、乳酸脱氢酶和己糖激酶的活性以及鳃中柠檬酸合成酶、磷酸果糖激酶和葡萄糖6-磷酸脱氢酶的活性在6个位点均无显著差异。对我们来说,它们是生物化学标记的候选者,因为这些酶的活性不受种群之间小的遗传变异以及环境中小的自然差异的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biochemical Marker of Environmental Changes in Antarctic Animals: Screening of Energy Metabolism Enzymes of Nacella Concinna for Monitoring of Intertidal Environment of Admiralty Bay, King George Island.
e Antarctic limpet Nacella concinna inhabits the intertidal shores of Admiralty Bay. e activities of energy metabolism enzymes of this species were determined in order to evaluate their potential as biochemical markers of intertidal environment changes. e limpets were collected on February 2011 at six intertidal sites at Admiralty Bay: Ullman Point, Botany Point, Punta Plaza, Hennequin Point, Brazilian Refuge 2 and in front of the oil tank of Brazilian Antarctic Station Comandante Ferraz. e enzyme activities were undertaken on the gills and foot tissues of these limpets. ere were no signi cant di erences in the activities of malate dehydrogenase, lactate dehydrogenase and hexokinase from foot tissue and citrate synthase, phosphofrutokinase and glucose 6-phosphate dehydrogenase from gills in samples from the six sites. us, they are candidates for biochemical markers because these enzyme activities are not a ected by small genetic variations between populations, as well as small natural di erences in the environment.
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