Nur Kusmiyati, Y. Puspitasari, U. Utami, Anggeria Oktavisa Denta
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引用次数: 1
摘要
外多糖因能改善宿主免疫系统而受到广泛关注。胞外多糖是一种由细胞外微生物产生和分泌的多糖,通常存在于细菌结构的外部。来自人类母乳的干酪乳杆菌群被认为具有产生外多糖的能力。本研究的目的是研究从母乳中分离出的干酪乳杆菌群的外多糖。采用的方法包括重量法、苯酚硫酸法和傅里叶变换红外(FTIR)法。结果表明,干酪乳杆菌组能产生胞外多糖,且胞外多糖总糖含量较高。副干酪乳杆菌的胞外多糖和总糖含量最高,分别为3660 mg/L和80.6%。FTIR结果表明,该菌胞外多糖具有羟基官能团O- h (3425.76 ~ 3295.98 cm-1)、甲基C- h (2930.86 ~ 2856.70 cm-1)、羰基C=O (1660.11 ~ 1647.27 cm-1)、C- h (1456.16 ~ 1373.44 cm-1)和C-O-C醚(1071.08 ~ 1056.82 cm-1)等特征。由于FTIR分析表明干酪乳杆菌群可以产生外多糖,因此它作为益生菌具有更大的潜力。
Analysis of Exopolysaccharides in Lactobacillus casei group Probiotics from Human Breast Milk
Exopolysaccharides get a lot of attention because they can improve the host immune system. Exopolysaccharide is a polysaccharide that is produced and secreted from microbes outside the cell, usually found on the outside of the bacterial structure. The Lactobacillus casei group from human breast milk is thought to have the ability to produce exopolysaccharides. The purpose of the study was to examine the exopolysaccharide of the L. casei group that was isolated from breast milk. The methods used include the gravimetric, the phenol-sulfuric acid and the Fourier Transform Infra-Red (FTIR). The results showed that the L. casei group could produce exopolysaccharides, and had high exopolysaccharide total sugar content. Lactobacillus paracasei had the highest exopolysaccharide and total sugar content of 3660 mg/L and 80.6%, respectively. The FTIR results of the L. casei group exopolysaccharides showed the presence of hydroxyl functional groups O-H (3425.76-3295.98 cm-1), methyl C-H (2930.86-2856.70 cm-1), carbonyl C=O (1660.11-1647.27 cm-1), C-H (1456.16-1373.44 cm-1) and C-O-C ether (1071.08-1056.82 cm-1) which are specific characters of exopolysaccharides. Since the FTIR profile demonstrates that the L. casei group can produce exopolysaccharides, it has greater potential as a a probiotic.