Synthesis, Characterization of substituted 4-(bromomethyl)-N-(4-ethyl-6-methylpyrimidin-2-yl)benzamide Derivatives and Evaluation of their Anti-microbial activity.

The substituted 4-(bromomethyl)-N-(4-ethyl-6-methylpyrimidin-2-yl) benzamide derivatives 6ak has been synthesis from reaction in between substituted 4-ethyl-6-methylpyrimidin-2-amine and 4-(bromomethyl)benzoyl bromide 5 in presence of KOH. Type of aldol condensation reaction in between aldehydes and ketones to form α,β-Unsaturated carbonyl compounds3 this compound has been reacted with guanidine in presence of dry alcohol to convert 4-ethyl-6methylpyrimidin-2-amine4.Chemical structures of all the new compounds were established by IR, 1H, 13C NMR, MS and elemental data. The compounds 6a-k were evaluated for their antibacterial activity against Gram-positive bacteria viz. Bacillus subtilis, Bacillus sphaericus and Staphylococcus aureus, and three Gram-negative bacteria viz. Pseudomonas aeruginosa, Klebsiella aerogenes and Chromobacterium violaceum and also evaluate their antifungal activity against Candida albicans (C.albicans)(ATCC 10231), Aspergillus fumigates(A.fumigatus) (HIC 6094), Trichophyton rubrum(T. rubrum) (IFO 9185), and Trichophyton mentagrophytes(T. mentagrophytes) (IFO 40996) Amongst them, compounds containing [3-hydrophenyl] moiety 6d, [3-chlorophenyl] moiety 6f and [4-nitrophenyl] moiety 6h showed significant antibacterial and antifungal activity, almost equal/more than the activity of the standard drugs Streptomycin and Amphotericin-B. Further, the compounds 6a-k were also screened for Most of these new compounds showed appreciable activity against test bacteria and fungi and emerged as potential molecules for further development.