{"title":"工艺参数对绿色木霉(MTCC 800)固态发酵产纤维素酶的影响","authors":"P. Kakde, S. Aithal","doi":"10.58537/angrau1","DOIUrl":null,"url":null,"abstract":"In this study, the effect of physico-chemical parameters on the production of cellulase from Trichoderma viride (MTCC 800) were examined using five different agricultural residues namely banana peel, groundnut shell, corn cob, sugarcane bagasse, and pigeonpea stalk. The process parameters examined were pretreatment method, supplementation media, initial media pH, incubation period, temperature, and leaching agent. Among the parameters studied, maximum cellulase production was obtained on all the substrates treated with 1 N H2SO4 supplemented with Mandel’s medium of pH 5.5-6 after 6-8 days of incubation at 30-36°C and 0.05 M phosphate buffer (pH 7.0) as a leaching agent.","PeriodicalId":220721,"journal":{"name":"THE JOURNAL OF RESEARCH ANGRAU","volume":"3 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"INFLUENCE OF PROCESS PARAMETERS ON CELLULASE PRODUCTION BY Trichoderma viride (MTCC 800) EMPLOYING SOLID STATE FERMENTATION\",\"authors\":\"P. Kakde, S. Aithal\",\"doi\":\"10.58537/angrau1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, the effect of physico-chemical parameters on the production of cellulase from Trichoderma viride (MTCC 800) were examined using five different agricultural residues namely banana peel, groundnut shell, corn cob, sugarcane bagasse, and pigeonpea stalk. The process parameters examined were pretreatment method, supplementation media, initial media pH, incubation period, temperature, and leaching agent. Among the parameters studied, maximum cellulase production was obtained on all the substrates treated with 1 N H2SO4 supplemented with Mandel’s medium of pH 5.5-6 after 6-8 days of incubation at 30-36°C and 0.05 M phosphate buffer (pH 7.0) as a leaching agent.\",\"PeriodicalId\":220721,\"journal\":{\"name\":\"THE JOURNAL OF RESEARCH ANGRAU\",\"volume\":\"3 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"THE JOURNAL OF RESEARCH ANGRAU\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.58537/angrau1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"THE JOURNAL OF RESEARCH ANGRAU","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.58537/angrau1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
以香蕉皮、花生壳、玉米芯、甘蔗渣和木豌豆秸秆为原料,研究了不同理化参数对绿色木霉(MTCC 800)生产纤维素酶的影响。考察了预处理方法、添加介质、初始介质pH、孵育时间、温度和浸出剂等工艺参数。在所研究的参数中,在30-36℃条件下,以0.05 M磷酸盐缓冲液(pH 7.0)作为浸出剂,1 N H2SO4加pH为5.5-6的Mandel培养基培养6-8天后,所有底物的纤维素酶产量均达到最高。
INFLUENCE OF PROCESS PARAMETERS ON CELLULASE PRODUCTION BY Trichoderma viride (MTCC 800) EMPLOYING SOLID STATE FERMENTATION
In this study, the effect of physico-chemical parameters on the production of cellulase from Trichoderma viride (MTCC 800) were examined using five different agricultural residues namely banana peel, groundnut shell, corn cob, sugarcane bagasse, and pigeonpea stalk. The process parameters examined were pretreatment method, supplementation media, initial media pH, incubation period, temperature, and leaching agent. Among the parameters studied, maximum cellulase production was obtained on all the substrates treated with 1 N H2SO4 supplemented with Mandel’s medium of pH 5.5-6 after 6-8 days of incubation at 30-36°C and 0.05 M phosphate buffer (pH 7.0) as a leaching agent.