博来霉素对小鼠成纤维细胞卫星DNA的影响。

Zeitschrift fur Krebsforschung und klinische Onkologie. Cancer research and clinical oncology Pub Date : 1978-10-30 DOI:10.1007/BF00461654

H Madreiter, R Osieka, C Wittekind, P Kaden, C Mittermayer

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引用次数: 2

摘要

研究了博来霉素对小鼠核DNA半保存复制的影响。在5-溴脱氧尿嘧啶(25 mg/l培养基)中培养异步分裂小鼠成纤维细胞(l -cells) 18 h，氯化铯密度梯度离心后，细胞核DNA出现密度分别为1.722、1.752和1.761 kg/l的杂交DNA条带。然而，在暴露于博莱霉素(100 mg/l)的细胞中，卫星DNA的复制比主带DNA的复制受到更强烈的抑制;首先，在1.761 kg/l的浓度下，富胸腺嘧啶杂交双相不再被检测到。

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Bleomycin: effect on satellite DNA in mouse fibroblasts.

The effect of Bleomycin on the semiconservative replication of mouse nuclear DNA has been studied. When asynchronously dividing mouse fibroblasts (L-cells) were grown in the presence of 5-bromodeoxyuridine (25 mg/l medium) for 18 h, three hybrid DNA bands with densities of 1.722, 1.752, and 1.761 kg/l appeared after caesium chloride density gradient centrifugation of nuclear DNA. In cells exposed to Bleomycin (100 mg/l) however, replication of satellite DNA is more strongly inhibited than is the replication of the main band DNA; preferentially the thymidinerich hybrid duplex at 1.761 kg/l could no longer be detected.

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Zeitschrift fur Krebsforschung und klinische Onkologie. Cancer research and clinical oncology

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