Amr O. Abdelkareem M.B.B.Ch., M.D. , Sahar M. Gebril M.D. , Faten F. AbdelHafez M.D. , Jefferson Terry M.D., Ph.D. , Mohamed A. Bedaiwy M.D., Ph.D.
{"title":"复发性妊娠丢失的整倍体和非整倍体绒毛膜蜕膜组织中的Kisspeptin和Kisspeptin受体免疫反应性","authors":"Amr O. Abdelkareem M.B.B.Ch., M.D. , Sahar M. Gebril M.D. , Faten F. AbdelHafez M.D. , Jefferson Terry M.D., Ph.D. , Mohamed A. Bedaiwy M.D., Ph.D.","doi":"10.1016/j.xfss.2022.10.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p><span>To study choriodecidual immunoreactivity<span> of kisspeptin<span> (KISS1) and its receptor (KISS1R) in recurrent pregnancy loss (RPL) due to </span></span></span>aneuploidy<span> (AnE) and unexplained (UE) RPL in comparison to control elective abortions (EAbs).</span></p></div><div><h3>Design</h3><p>This is a case-control study.</p></div><div><h3>Setting</h3><p>Tertiary care facility and affiliated research institute.</p></div><div><h3>Patient(s)</h3><p>Patients with either UE RPL (n = 10) or RPL due to AnE (n = 10) vs. a control group of patients who underwent EAb (n = 10).</p></div><div><h3>Intervention(s)</h3><p>Immunohistochemistry of archived choriodecidual tissue samples.</p></div><div><h3>Main Outcome Measure(s)</h3><p>Histoscores of KISS1 and KISS1R immunoreactivity in the syncytiotrophoblast<span><span> (SyT), cytotrophoblast (CyT), decidual glands (DeGs), and decidual </span>stroma (DeS) across the 3 study groups.</span></p></div><div><h3>Result(s)</h3><p>There was no difference in both maternal and gestational ages among the 3 groups. Kisspeptin immunoreactivity was similar in the SyT, CyT, DeGs, and DeS of all groups. Similarly, KISS1R expression was not different in the DeGs or DeS among all study groups. In addition, there was no difference in KISS1R immunoreactivity in the SyTs and CyTs between patients with RPL due to AnE and those with UE RPL. However, KISS1R was significantly lower in the SyT and CyT of patients with RPL due to AnE and UE RPL than in those who underwent EAb.</p></div><div><h3>Conclusion(s)</h3><p>The expression of KISS1R is lower in the chorionic tissues<span> of euploid (unexplained) and aneuploid RPLs than in the control group. The current results broaden our understanding of the role played by KISS1 and KISS1R in early placentation. Further investigation is necessary to determine whether KISS1 activity is the cause or a sequel of defective placentation.</span></p></div>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":"4 1","pages":"Pages 56-64"},"PeriodicalIF":0.0000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Kisspeptin and kisspeptin receptor immunoreactivity in euploid and aneuploid choriodecidual tissues of recurrent pregnancy losses\",\"authors\":\"Amr O. Abdelkareem M.B.B.Ch., M.D. , Sahar M. Gebril M.D. , Faten F. AbdelHafez M.D. , Jefferson Terry M.D., Ph.D. , Mohamed A. Bedaiwy M.D., Ph.D.\",\"doi\":\"10.1016/j.xfss.2022.10.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p><span>To study choriodecidual immunoreactivity<span> of kisspeptin<span> (KISS1) and its receptor (KISS1R) in recurrent pregnancy loss (RPL) due to </span></span></span>aneuploidy<span> (AnE) and unexplained (UE) RPL in comparison to control elective abortions (EAbs).</span></p></div><div><h3>Design</h3><p>This is a case-control study.</p></div><div><h3>Setting</h3><p>Tertiary care facility and affiliated research institute.</p></div><div><h3>Patient(s)</h3><p>Patients with either UE RPL (n = 10) or RPL due to AnE (n = 10) vs. a control group of patients who underwent EAb (n = 10).</p></div><div><h3>Intervention(s)</h3><p>Immunohistochemistry of archived choriodecidual tissue samples.</p></div><div><h3>Main Outcome Measure(s)</h3><p>Histoscores of KISS1 and KISS1R immunoreactivity in the syncytiotrophoblast<span><span> (SyT), cytotrophoblast (CyT), decidual glands (DeGs), and decidual </span>stroma (DeS) across the 3 study groups.</span></p></div><div><h3>Result(s)</h3><p>There was no difference in both maternal and gestational ages among the 3 groups. Kisspeptin immunoreactivity was similar in the SyT, CyT, DeGs, and DeS of all groups. Similarly, KISS1R expression was not different in the DeGs or DeS among all study groups. In addition, there was no difference in KISS1R immunoreactivity in the SyTs and CyTs between patients with RPL due to AnE and those with UE RPL. However, KISS1R was significantly lower in the SyT and CyT of patients with RPL due to AnE and UE RPL than in those who underwent EAb.</p></div><div><h3>Conclusion(s)</h3><p>The expression of KISS1R is lower in the chorionic tissues<span> of euploid (unexplained) and aneuploid RPLs than in the control group. The current results broaden our understanding of the role played by KISS1 and KISS1R in early placentation. Further investigation is necessary to determine whether KISS1 activity is the cause or a sequel of defective placentation.</span></p></div>\",\"PeriodicalId\":73012,\"journal\":{\"name\":\"F&S science\",\"volume\":\"4 1\",\"pages\":\"Pages 56-64\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"F&S science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666335X22000647\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"F&S science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666335X22000647","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Kisspeptin and kisspeptin receptor immunoreactivity in euploid and aneuploid choriodecidual tissues of recurrent pregnancy losses
Objective
To study choriodecidual immunoreactivity of kisspeptin (KISS1) and its receptor (KISS1R) in recurrent pregnancy loss (RPL) due to aneuploidy (AnE) and unexplained (UE) RPL in comparison to control elective abortions (EAbs).
Design
This is a case-control study.
Setting
Tertiary care facility and affiliated research institute.
Patient(s)
Patients with either UE RPL (n = 10) or RPL due to AnE (n = 10) vs. a control group of patients who underwent EAb (n = 10).
Intervention(s)
Immunohistochemistry of archived choriodecidual tissue samples.
Main Outcome Measure(s)
Histoscores of KISS1 and KISS1R immunoreactivity in the syncytiotrophoblast (SyT), cytotrophoblast (CyT), decidual glands (DeGs), and decidual stroma (DeS) across the 3 study groups.
Result(s)
There was no difference in both maternal and gestational ages among the 3 groups. Kisspeptin immunoreactivity was similar in the SyT, CyT, DeGs, and DeS of all groups. Similarly, KISS1R expression was not different in the DeGs or DeS among all study groups. In addition, there was no difference in KISS1R immunoreactivity in the SyTs and CyTs between patients with RPL due to AnE and those with UE RPL. However, KISS1R was significantly lower in the SyT and CyT of patients with RPL due to AnE and UE RPL than in those who underwent EAb.
Conclusion(s)
The expression of KISS1R is lower in the chorionic tissues of euploid (unexplained) and aneuploid RPLs than in the control group. The current results broaden our understanding of the role played by KISS1 and KISS1R in early placentation. Further investigation is necessary to determine whether KISS1 activity is the cause or a sequel of defective placentation.