味噌中Zygosaccharomyces sp.酵母菌的分离与鉴定。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Tomoko Fujiwara, Atsuko Matsura, Momoka Fukuda, Katsuaki Kuroki, Tomoo Ogata
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引用次数: 0

摘要

目前,人们对用于生产味噌和酱油的耐盐酵母菌株非常感兴趣。自从从日本味噌中分离到Zygosaccharomyces sp.菌株以来,已经在发酵食品中发现了几种杂交菌株。研究表明,原始Zygosaccharomyces sp.酵母菌株的活跃交配型位点位于t -亚基因组序列和p -亚基因组序列之间。本研究从日本广岛县的5家味噌工厂分离得到32株耐盐酵母酵母。流式细胞术分析显示,27株为二倍体,5株为单倍体。PCR分析表明,27株二倍体菌株具有与60年前从miso中分离的酵母株相同的活性交配型(MAT)位点的染色体结构。另外,27株二倍体菌株为异源二倍体,即rouxii与亲缘种的自然杂种,而5株单倍体菌株均为rouxii。我们发现,从miso分离的酵母菌株与相反交配型的酵母菌株在氮饥饿条件下共培养时,细胞形态发生了变化。活性交合型位点的DNA序列和共培养后细胞形态变化的结果与交合实验中各品系的交合型一致。这些发现将对未来味噌和酱油的生产有所帮助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of Zygosaccharomyces sp. yeast strains from miso.

There is currently great interest in the salt-tolerant yeast strains used to produce miso and soy sauce. Since the isolation of Zygosaccharomyces sp. strain from Japanese miso more than 60 years, several hybrid strains have been identified in fermented foods. Studies have shown that the active mating-type locus of the original Zygosaccharomyces sp. yeast strain is located between the T-subgenome sequence and the P-subgenome sequence. In this study, 32 salt-tolerant Zygosaccharomyces sp. yeast strains were isolated from five miso factories in Hiroshima Prefecture, Japan. Analysis by flow cytometry revealed that 27 strains were diploid and five strains were haploid. PCR analysis indicated that the 27 diploid strains had the same chromosomal structure of the active mating-type (MAT) locus as the original yeast strain isolated from miso 60 years ago. In addition, the 27 diploid strains were allodiploid, namely, natural hybrids of Z. rouxii and a related species, while the five haploid strains were all Z. rouxii. We found that cells of yeast strains isolated from miso changed morphologically when co-cultured with a yeast strain of opposite mating-type under nitrogen starvation conditions. The DNA sequence of the active mating-type locus and the results of cell morphology changes by co-culture were consistent with the mating type of each strain shown in the mating experiments. These findings will be useful for the future production of miso and soy sauce.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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