阴道加德纳菌分离株的特征:分支、生物膜形成和细胞因子刺激之间的相关性。

IF 1.5 4区医学 Q4 MICROBIOLOGY

New Microbiologica Pub Date : 2023-02-01

Sara Morselli, Melissa Salvo, Claudio Foschi, Tiziana Lazzarotto, Simone Ambretti, Antonella Marangoni

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引用次数: 0

摘要

我们对从细菌性阴道病女性中分离的61株阴道加德纳菌(GV)进行了鉴定。GV进化枝1最常见(52.5%)，其次是进化枝4(36.1%)。所有菌株对氨苄西林和克林霉素均敏感，对甲硝唑和四环素的耐药率分别为96.7%和6.6%。分支4中的分离株往往具有最高的形成生物膜的能力。对甲硝唑和四环素耐药菌株均为中高生物膜生产者。所有GV分支均显著上调HeLa细胞产生的促炎细胞因子，尤其是IL-8和IL-6。与其他分支相比，Clade 4诱导IL-1β的产生显著增加。

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Characterization of Gardnerella vaginalis isolates: correlations among clades, biofilm formation and cytokine stimulation.

We characterized 61 Gardnerella vaginalis (GV) strains isolated from women with bacterial vaginosis. GV clade 1 was the most commonly found (52.5%), followed by clade 4 (36.1%). All the strains were susceptible to ampicillin and clindamycin, whereas 96.7% and 6.6% of strains showed metronidazole and tetracycline resistance, respectively. Isolates within clade 4 tended to possess the highest ability to form biofilm. Strains resistant to metronidazole and tetracycline were all intermediate or high biofilm producers. All GV clades significantly upregulated the production of pro-inflammatory cytokines by HeLa cells, especially IL-8 and IL-6. Clade 4 induced a significantly higher production of IL-1β compared to other clades.

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来源期刊

New Microbiologica 生物-微生物学

CiteScore

2.20

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The publication, diffusion and furtherance of research and study on all aspects of basic and clinical Microbiology and related fields are the chief aims of the journal.