用于植入骨分析的骨质疏松症实验模型的快速建立和验证。

IF 1.3 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Wenxue Wang, Hao Xu, Baodong Zhao, Xin Li
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引用次数: 0

摘要

骨质疏松症是一种日益普遍的疾病。开发一种类似骨质疏松症的实验动物模型对骨质疏松症种植体周围成骨的研究具有重要意义。本作者旨在建立一种用于种植体骨分析的骨质疏松兔快速建模方法,并验证这些模型是否会影响种植体骨整合。本研究包括29只雌性新西兰兔(年龄:5至6个月)。麻醉期间丢失了两只兔子。在剩下的27只兔子中,18只接受了卵巢切除术,9只接受地塞米松注射8周(OVX+D组),9只注射等量生理盐水(OVX组)。作为对照组,其余9只兔子进行假手术,并接受等量的生理盐水(sham组)。然后,检测骨代谢的血清生化标志物,并进行密度测定。然后将植入物放置在每只兔子的胫骨中。在愈合4、8和12周后获得骨样本(包括植入物),并进行组织学和组织形态计量学分析。结果显示,OVX+D组的骨密度(BMD)较基线降低了32%。OVX+D组的骨密度显著低于SHAM和OVX组。此外,OVX+D组的碱性磷酸酶(ALP)血药浓度显著升高。骨质疏松兔的骨整合显著降低,其特征是骨形成减慢和骨与植入物接触(BIC)减少。卵巢切除术和地塞米松注射相结合可以在短期内通过实验诱导兔骨质疏松症,这可以作为研究骨质疏松症下植入物骨整合的合适动物模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rapid Establishment and Validation of an Experimental Model of Osteoporosis for Implant-Bone Analysis.

Osteoporosis is an increasingly prevalent disease. The development of an osteoporosis-like experimental animal model is of great importance for the study of peri-implant osteogenesis in osteoporosis. The present authors aimed to establish a rapid modeling method of osteoporotic rabbits for implant-bone analysis and validate whether the models can affect the implant osseointegration. The present study included 29 female New Zealand rabbits (age: 5 to 6 months). Two rabbits were lost during anesthetization. Of the remaining 27 rabbits, 18 received an ovariectomy, with 9 receiving dexamethasone injections for 8 weeks (OVX+D group) and 9 receiving equivalent-volume saline injections (OVX group). As a control group, the remaining 9 rabbits were sham-operated and received an equivalent volume of normal saline (SHAM group). Then, serum biochemical markers of bone metabolism were detected and densitometric measurements were performed. Implants were then placed in the tibias of each rabbit. Bone samples (including implants) were obtained after 4, 8, and 12 weeks of healing and were subjected to histologic and histomorphometric analyses. The results showed that the OVX+D group experienced a 32% reduction in bone mineral density (BMD) from baseline. The BMD of the OVX+D group was significantly lower than that of the SHAM and OVX groups. Moreover, alkaline phosphatase (ALP) blood concentrations of in the OVX+D group were increased significantly. The osteoporotic rabbits exhibited marked decreases in osseointegration, characterized by slowed bone formation and decreased bone-to-implant contact (BIC). The combination of an ovariectomy and dexamethasone injections could experimentally induce osteoporosis in rabbits in the short term, which can be used as an appropriate animal model to study the osseointegration of implants under osteoporosis.

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来源期刊
CiteScore
2.90
自引率
6.20%
发文量
113
审稿时长
6-12 weeks
期刊介绍: The International Journal of Periodontics & Restorative Dentistry will publish manuscripts concerned with all aspects of clinical periodontology, restorative dentistry, and implantology. This includes pertinent research as well as clinical methodology (their interdependence and relationship should be addressed where applicable); proceedings of relevant symposia or conferences; and quality review papers. Original manuscripts are considered for publication on the condition that they have not been published or submitted for publication elsewhere.
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