Halofuginone抑制子宫平滑肌瘤细胞增殖和AKT/mTORC1信号通路。

IF 1.8 4区 生物学 Q4 CELL BIOLOGY
Jing Lin, Xiaochun Wu
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引用次数: 0

摘要

本研究旨在探讨抗纤维化药物氟化酮对子宫平滑肌瘤细胞的影响。测定子宫平滑肌细胞(UtSMC)和子宫上皮细胞(ulelt3)的存活率。流式细胞术检测细胞周期分布及凋亡情况。观察氟醌对AKT/mTOR通路状态的影响。采用异种移植动物模型,探讨了氟醌在体内的作用。Halofuginone抑制ELT3细胞增殖呈剂量依赖性,对UtSMC细胞无明显影响。Halofuginone抑制细胞周期进程,促进ELT3细胞凋亡呈剂量依赖性。20 nM氟化酮处理后,p-AKT/AKT和p-p70S6/p70S6均显著降低。此外,在异种移植肿瘤动物模型中,halofuginone还能抑制ELT3肿瘤的生长。本研究表明,halofuginone抑制ULs细胞增殖,对正常平滑肌细胞的副作用低,同时AKT/mTOR信号通路失活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Halofuginone inhibits cell proliferation and AKT/mTORC1 signaling in uterine leiomyoma cells.

The present study aimed to explore the effects of antifibrotic agent halofuginone on uterine leiomyomas (ULs) cells. The survival of the uterine smooth muscle (UtSMC) cells and UL ELT3 cells were measured. Flow cytometry was used to assess the cell cycle distribution and apoptosis. Effects of halofuginone on the state of AKT/mTOR pathway were evaluated. Xenograft animal model was applied to explore the effects of halofuginone in vivo. Halofuginone inhibited the proliferation of ELT3 cells dose-dependently without obvious influence on UtSMC cells. Halofuginone suppressed cell cycle progression and promoted apoptosis of ELT3 cells dose-dependently. Also, p-AKT/AKT and p-p70S6/p70S6 were significantly lowered after treatment with 20 nM halofuginone. Additionally, halofuginone reduced ELT3 tumor growth in xenograft tumor animal model. The present study illustrates that halofuginone inhibits cell proliferation of ULs with low side effects on normal smooth muscle cells, and AKT/mTOR signaling pathway was inactivated meanwhile.

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来源期刊
Growth factors
Growth factors 生物-内分泌学与代谢
CiteScore
2.60
自引率
0.00%
发文量
20
审稿时长
>12 weeks
期刊介绍: Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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