Ina Prade , Michaela Schröpfer , Caroline Seidel , Claudia Krumbiegel , Tina Hille , Frank Sonntag , Stephen Behrens , Florian Schmieder , Birgit Voigt , Michael Meyer
{"title":"人内皮细胞在直接挤压打印制备的独立胶原中空纤维中形成内皮","authors":"Ina Prade , Michaela Schröpfer , Caroline Seidel , Claudia Krumbiegel , Tina Hille , Frank Sonntag , Stephen Behrens , Florian Schmieder , Birgit Voigt , Michael Meyer","doi":"10.1016/j.bbiosy.2022.100067","DOIUrl":null,"url":null,"abstract":"<div><p>Fiber-shaped materials have great potential for tissue engineering applications as they provide structural support and spatial patterns within a three-dimensional construct. Here we demonstrate the fabrication of mechanically stable, meter-long collagen hollow filaments by a direct extrusion printing process. The fibres are permeable for oxygen and proteins and allow cultivation of primary human endothelial cells (ECs) at the inner surface under perfused conditions. The cells show typical characteristics of a well-organized EC lining including VE-cadherin expression, cellular response to flow and ECM production. The results demonstrate that the collagen tubes are capable of creating robust soft tissue filaments. The mechanical properties and the biofunctionality of these collagen hollow filaments facilitate the engineering of prevascularised tissue engineering constructs.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"8 ","pages":"Article 100067"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/a6/main.PMC9934428.pdf","citationCount":"0","resultStr":"{\"title\":\"Human endothelial cells form an endothelium in freestanding collagen hollow filaments fabricated by direct extrusion printing\",\"authors\":\"Ina Prade , Michaela Schröpfer , Caroline Seidel , Claudia Krumbiegel , Tina Hille , Frank Sonntag , Stephen Behrens , Florian Schmieder , Birgit Voigt , Michael Meyer\",\"doi\":\"10.1016/j.bbiosy.2022.100067\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Fiber-shaped materials have great potential for tissue engineering applications as they provide structural support and spatial patterns within a three-dimensional construct. Here we demonstrate the fabrication of mechanically stable, meter-long collagen hollow filaments by a direct extrusion printing process. The fibres are permeable for oxygen and proteins and allow cultivation of primary human endothelial cells (ECs) at the inner surface under perfused conditions. The cells show typical characteristics of a well-organized EC lining including VE-cadherin expression, cellular response to flow and ECM production. The results demonstrate that the collagen tubes are capable of creating robust soft tissue filaments. The mechanical properties and the biofunctionality of these collagen hollow filaments facilitate the engineering of prevascularised tissue engineering constructs.</p></div>\",\"PeriodicalId\":72379,\"journal\":{\"name\":\"Biomaterials and biosystems\",\"volume\":\"8 \",\"pages\":\"Article 100067\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/a6/main.PMC9934428.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomaterials and biosystems\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666534422000290\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomaterials and biosystems","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666534422000290","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Human endothelial cells form an endothelium in freestanding collagen hollow filaments fabricated by direct extrusion printing
Fiber-shaped materials have great potential for tissue engineering applications as they provide structural support and spatial patterns within a three-dimensional construct. Here we demonstrate the fabrication of mechanically stable, meter-long collagen hollow filaments by a direct extrusion printing process. The fibres are permeable for oxygen and proteins and allow cultivation of primary human endothelial cells (ECs) at the inner surface under perfused conditions. The cells show typical characteristics of a well-organized EC lining including VE-cadherin expression, cellular response to flow and ECM production. The results demonstrate that the collagen tubes are capable of creating robust soft tissue filaments. The mechanical properties and the biofunctionality of these collagen hollow filaments facilitate the engineering of prevascularised tissue engineering constructs.