利用基因编码传感器对神经活动、神经化学动态和药物特异性受体构象进行荧光成像。

IF 12.1 1区 医学 Q1 NEUROSCIENCES
Annual review of neuroscience Pub Date : 2022-07-08 Epub Date: 2022-03-22 DOI:10.1146/annurev-neuro-110520-031137
Chunyang Dong, Yu Zheng, Kiran Long-Iyer, Emily C Wright, Yulong Li, Lin Tian
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引用次数: 0

摘要

荧光成像技术的最新进展允许以前所未有的分辨率对行为动物的神经活动和神经化学物质释放动态进行大规模记录。利用高度优化的基因编码指示器进行钙成像,可在细胞和亚细胞分辨率上以中观视角观察基因定义的群体的神经活动。经过严格改进的电压传感器和显微镜可对不同脑区的群体神经元进行稳健的尖峰成像。此外,在过去几年中,蛋白质工程学的最新研究成果开发出了神经递质和神经调节剂传感器。在此,我们将讨论这些基因编码荧光指示剂在报告不同生物系统中神经活动对各种行为的反应以及在药物发现中的开发和应用。我们还报告了一个指导传感器选择和优化的简单模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fluorescence Imaging of Neural Activity, Neurochemical Dynamics, and Drug-Specific Receptor Conformation with Genetically Encoded Sensors.

Fluorescence Imaging of Neural Activity, Neurochemical Dynamics, and Drug-Specific Receptor Conformation with Genetically Encoded Sensors.

Recent advances in fluorescence imaging permit large-scale recording of neural activity and dynamics of neurochemical release with unprecedented resolution in behaving animals. Calcium imaging with highly optimized genetically encoded indicators provides a mesoscopic view of neural activity from genetically defined populations at cellular and subcellular resolutions. Rigorously improved voltage sensors and microscopy allow for robust spike imaging of populational neurons in various brain regions. In addition, recent protein engineering efforts in the past few years have led to the development of sensors for neurotransmitters and neuromodulators. Here, we discuss the development and applications of these genetically encoded fluorescent indicators in reporting neural activity in response to various behaviors in different biological systems as well as in drug discovery. We also report a simple model to guide sensor selection and optimization.

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来源期刊
Annual review of neuroscience
Annual review of neuroscience 医学-神经科学
CiteScore
25.30
自引率
0.70%
发文量
29
期刊介绍: The Annual Review of Neuroscience is a well-established and comprehensive journal in the field of neuroscience, with a rich history and a commitment to open access and scholarly communication. The journal has been in publication since 1978, providing a long-standing source of authoritative reviews in neuroscience. The Annual Review of Neuroscience encompasses a wide range of topics within neuroscience, including but not limited to: Molecular and cellular neuroscience, Neurogenetics, Developmental neuroscience, Neural plasticity and repair, Systems neuroscience, Cognitive neuroscience, Behavioral neuroscience, Neurobiology of disease. Occasionally, the journal also features reviews on the history of neuroscience and ethical considerations within the field.
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