{"title":"两步制备整体色谱法纯化功能性基因转移剂。","authors":"Evan Langille, Christina S Bottaro, Andrew S Lang","doi":"10.1089/phage.2022.0035","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Gene transfer agents (GTAs) are phage-like particles that transfer cellular genomic DNA between cells. A hurdle faced in studying GTA function and interactions with cells is the difficulty in obtaining pure and functional GTAs from cultures.</p><p><strong>Materials and methods: </strong>We used a novel two-step method for purification of GTAs from <i>R. capsulatus</i> by monolithic chromatography.</p><p><strong>Results: </strong>Our efficient and simple process had advantages compared to previous approaches. The purified GTAs retained gene transfer activity and the packaged DNA could be used for further studies.</p><p><strong>Conclusions: </strong>This method is applicable to GTAs produced by other species and small phages, and could be useful for therapeutic applications.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"3 4","pages":"194-203"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917305/pdf/","citationCount":"0","resultStr":"{\"title\":\"Purification of Functional Gene Transfer Agents Using Two-Step Preparative Monolithic Chromatography.\",\"authors\":\"Evan Langille, Christina S Bottaro, Andrew S Lang\",\"doi\":\"10.1089/phage.2022.0035\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Gene transfer agents (GTAs) are phage-like particles that transfer cellular genomic DNA between cells. A hurdle faced in studying GTA function and interactions with cells is the difficulty in obtaining pure and functional GTAs from cultures.</p><p><strong>Materials and methods: </strong>We used a novel two-step method for purification of GTAs from <i>R. capsulatus</i> by monolithic chromatography.</p><p><strong>Results: </strong>Our efficient and simple process had advantages compared to previous approaches. The purified GTAs retained gene transfer activity and the packaged DNA could be used for further studies.</p><p><strong>Conclusions: </strong>This method is applicable to GTAs produced by other species and small phages, and could be useful for therapeutic applications.</p>\",\"PeriodicalId\":74428,\"journal\":{\"name\":\"PHAGE (New Rochelle, N.Y.)\",\"volume\":\"3 4\",\"pages\":\"194-203\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917305/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PHAGE (New Rochelle, N.Y.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/phage.2022.0035\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/12/19 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2022.0035","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/12/19 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Purification of Functional Gene Transfer Agents Using Two-Step Preparative Monolithic Chromatography.
Background: Gene transfer agents (GTAs) are phage-like particles that transfer cellular genomic DNA between cells. A hurdle faced in studying GTA function and interactions with cells is the difficulty in obtaining pure and functional GTAs from cultures.
Materials and methods: We used a novel two-step method for purification of GTAs from R. capsulatus by monolithic chromatography.
Results: Our efficient and simple process had advantages compared to previous approaches. The purified GTAs retained gene transfer activity and the packaged DNA could be used for further studies.
Conclusions: This method is applicable to GTAs produced by other species and small phages, and could be useful for therapeutic applications.
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