先兆子痫患者胎盘SOD1基因异常甲基化及其表达和酶活性。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Saba Zakeri, Zohreh Rahimi, Nazanin Jalilian, Asad Vaisi-Raygani, Ali Rezvani, Zahra Dastafkan
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引用次数: 0

摘要

背景:氧化应激参与了子痫前期(PE)的发病机制。SOD1的失调可能参与PE的发病机制。我们检测并比较了PE妇女胎盘和母体血液中SOD1启动子区(PMR)的甲基化水平、基因表达和超氧化物歧化酶(SOD)的酶活性。方法:对来自PE和健康孕妇对照的140份血液样本和40份胎盘组织样本进行研究。研究并比较两组SOD1的PMR(Methylight PCR法)、表达(Real-time PCR)及其酶活性。结果:与对照组相比,患者胎盘组织中SOD1基因的PMR显著升高(P=0.008);该结果伴随着基因表达的降低和SOD酶活性的降低。同时,SOD1基因的PMR在患者的血液样本中没有显著变化(P=0.95),而SOD1的表达显著降低(SOD活性没有显著变化)。结论:SOD1基因的PMR和基因在胎盘组织中的表达发生了显著变化。研究结果强调了胎盘在妊娠并发症中的作用,并揭示了表观遗传学在先兆子痫发病机制中的重要调控途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aberrant Methylation of the SOD1 GENE, its Expression and Enzyme Activity in the Placenta of Patients with Preeclampsia.

Background: Oxidative stress is involved in the pathogenesis of preeclampsia (PE). Dysregulation of SOD1 may be involved in the pathogenesis of PE. We examined and compared the methylation level of the promoter region (PMR) of the SOD1, gene expression, and enzyme activity of superoxide dismutase (SOD) in both placenta and maternal blood in PE women.

Methods: A total of 140 blood samples and 40 placental tissue samples from PE and healthy pregnant controls were studied. The PMR of the SOD1 (Methylight PCR method), the expression (Real-time PCR), and its enzyme activity were investigated and compared in two groups.

Results: The PMR of the SOD1 gene in the placental tissue of the patients was significantly increased compared to the control group (P= 0.008); this result was accompanied by a decrease in the expression of the gene and a decrease in the activity of the SOD enzyme. Meanwhile, the PMR of the SOD1 gene did not significantly change in the blood samples of the patients (P= 0.95), while a significant decrease in the expression of SOD1 (without a significant change in the SOD activity) was observed.

Conclusion: The results showed significant changes in the PMR of the SOD1 gene and gene expression in placenta tissue. The results highlight the role of the placenta in complications during pregnancy and also revealed epigenetics as an important regulatory pathway in the pathogenesis of preeclampsia.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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