肥胖中的GPx-1基因变体(rs1050450):与女性肥胖风险和GPx活性的关系。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Avan Arif Ahmad, Zohreh Rahimi, Soheila Asadi, Asad Vaisi-Raygani, Maryam Kohsari
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引用次数: 0

摘要

背景:本研究旨在调查伊拉克苏莱曼尼一名肥胖库尔德人群的GPx-1基因多态性(rs1050450)、氧化应激和抗氧化参数水平以及脂质状况。方法:在一项病例对照研究中,134名肥胖受试者和131名BMI正常的健康人参与了研究。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测GPx-1基因多态性。使用光度法测定生化和氧化参数的水平。结果:与对照组相比,肥胖受试者的空腹血糖(FBS)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)水平显著升高。肥胖个体的高密度脂蛋白胆固醇(HDL-C)水平显著低于对照组。与对照组相比,肥胖组的GPx-1活性和总抗氧化能力(TAC)水平显著升高(P=0.006,Px-1TT基因型使女性肥胖风险增加了1.93倍(95%CI 1.04-3.58,P=0.036)。在总人群中,与CC+CT和CT基因型相比,存在TT时的GPx活性增加。结论:肥胖与FBS、TG、LDL-C、TAC和GPx活性显著升高和HDL-C水平显著降低有关。此外,我们发现GPx-1基因多态性与女性肥胖风险相关,并增加了GPx活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The GPx-1 Gene Variants (rs1050450) in Obesity: Association with the Risk of Obesity and the GPx Activity in Females.

Background: This study aimed to investigate the GPx-1 gene polymorphism (rs1050450), the level of oxidative stress and antioxidant parameters, and the lipid profile in an obese Kurdish population in Sulaimani, Iraq.

Methods: In a case-control study,134 obese subjects and 131 normal BMI healthy individuals participated. The GPx-1 gene polymorphism was assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The levels of biochemical and oxidative parameters were determined using photometric methods.

Results: The results showed that the fasting blood sugar (FBS), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) levels were significantly higher in obese subjects compared to the control group. Obese individuals had significantly lower levels of high-density lipoprotein cholesterol (HDL-C) than the controls. The GPx-1 activity and total antioxidant capacity (TAC) levels were significantly elevated in the obese group compared to the control group (P=0.006, and P<0.001, respectively). No significant difference was detected in genotype and allele frequencies of GPx-1 (rs1050450) between obese and normal BMI groups. However, the presence of the GPx-1 TT genotype enhanced the risk of obesity in females by 1.93-fold (95% CI 1.04-3.58, P=0.036). In the total population, the GPx activity increased in the presence of TT compared to CC+CT and CT genotypes.

Conclusion: The study indicated that obesity is linked to significantly higher levels of FBS, TG, LDL-C, TAC, and GPx activity and lower level of HDL-C. Also, we found the GPx-1 gene polymorphism was associated with the risk of obesity in females and increased the GPx activity.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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