V C S Alves, R A Lira, J M S Lima, R N Barbosa, D M Bento, E Barbier, E Bernard, C M Souza-Motta, J D P Bezerra
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We used morphological features and phylogenetic analyses of actin (<i>actA</i>), calmodulin (<i>cmdA</i>), internal transcribed spacer regions and intervening 5.8S rRNA (ITS), large subunit (LSU) rDNA, RNA polymerase II second largest subunit (<i>rpb2</i>), translation elongation factor 1-alpha (<i>tef1</i>), and β-tubulin (<i>tub2</i>) genes to identify these isolates. Forty-one species belonging to 17 genera of <i>Ascomycota</i> and two of <i>Basidiomycota</i> were identified, and the genus <i>Aspergillus</i> was most commonly observed in the cave (13 taxa). Twenty-four species were found in sediment (16 exclusives) and 25 species were found in air (17 exclusives). In this study, we introduced a new genus (<i>Pseudolecanicillium gen. nov</i>.) in the family <i>Cordycipitaceae</i> and six new species (14 % of the total taxa identified) of fungal isolates obtained from sediment and air: <i>Aspergillus lebretii sp. nov</i>., <i>Malbranchea cavernosa sp. nov</i>., <i>Pseudohumicola cecavii sp. nov</i>., <i>Pseudolecanicillium caatingaense sp. nov</i>., <i>Talaromyces cavernicola sp. nov</i>., and <i>Tritirachium brasiliense</i> <i>sp. nov</i>. In addition, we built a checklist of the fungal taxa reported from Brazilian caves. Our results highlight the contribution of Brazilian caves to the estimation of national and global fungal diversity. <b>Citation:</b> Alves VCS, Lira RA, Lima JMS, Barbosa RN, Bento DM, Barbier E, Bernard E, Souza-Motta CM, Bezerra JDP (2022). Unravelling the fungal darkness in a tropical cave: richness and the description of one new genus and six new species. <i>Fungal Systematics and Evolution</i> <b>10</b>: 139-167. doi: 10.3114/fuse.2022.10.06.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"10 ","pages":"139-167"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9875697/pdf/","citationCount":"0","resultStr":"{\"title\":\"Unravelling the fungal darkness in a tropical cave: richness and the description of one new genus and six new species.\",\"authors\":\"V C S Alves, R A Lira, J M S Lima, R N Barbosa, D M Bento, E Barbier, E Bernard, C M Souza-Motta, J D P Bezerra\",\"doi\":\"10.3114/fuse.2022.10.06\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Caves are special environments that harbour an incredible diversity of life, including fungal species. Brazilian caves have been demonstrated to be biodiversity hotspots for known and unknown fungal species. We investigated the richness of culturable fungi in a tropical cave in Brazil by isolating these microorganisms from the sediment and air. The fungal abundance of colony-forming units (CFUs) was 3 178 in sediment and 526 in air. We used morphological features and phylogenetic analyses of actin (<i>actA</i>), calmodulin (<i>cmdA</i>), internal transcribed spacer regions and intervening 5.8S rRNA (ITS), large subunit (LSU) rDNA, RNA polymerase II second largest subunit (<i>rpb2</i>), translation elongation factor 1-alpha (<i>tef1</i>), and β-tubulin (<i>tub2</i>) genes to identify these isolates. Forty-one species belonging to 17 genera of <i>Ascomycota</i> and two of <i>Basidiomycota</i> were identified, and the genus <i>Aspergillus</i> was most commonly observed in the cave (13 taxa). Twenty-four species were found in sediment (16 exclusives) and 25 species were found in air (17 exclusives). In this study, we introduced a new genus (<i>Pseudolecanicillium gen. nov</i>.) in the family <i>Cordycipitaceae</i> and six new species (14 % of the total taxa identified) of fungal isolates obtained from sediment and air: <i>Aspergillus lebretii sp. nov</i>., <i>Malbranchea cavernosa sp. nov</i>., <i>Pseudohumicola cecavii sp. nov</i>., <i>Pseudolecanicillium caatingaense sp. nov</i>., <i>Talaromyces cavernicola sp. nov</i>., and <i>Tritirachium brasiliense</i> <i>sp. nov</i>. In addition, we built a checklist of the fungal taxa reported from Brazilian caves. Our results highlight the contribution of Brazilian caves to the estimation of national and global fungal diversity. <b>Citation:</b> Alves VCS, Lira RA, Lima JMS, Barbosa RN, Bento DM, Barbier E, Bernard E, Souza-Motta CM, Bezerra JDP (2022). 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引用次数: 0
摘要
洞穴是一种特殊的环境，蕴藏着令人难以置信的生命多样性，包括真菌物种。巴西洞穴已被证明是已知和未知真菌物种的生物多样性热点。我们从沉积物和空气中分离出这些微生物，调查了巴西一个热带洞穴中可培养真菌的丰富程度。沉积物中的真菌菌落形成单位（CFU）数量为 3 178 个，空气中为 526 个。我们利用形态特征和肌动蛋白（actA）、钙调蛋白（cmdA）、内部转录间隔区和中间的 5.8S rRNA（ITS）、大亚基（LSU）rDNA、RNA 聚合酶 II 第二大亚基（rpb2）、翻译延伸因子 1-α（tef1）和 β-微管蛋白（tub2）基因的系统发育分析来鉴定这些分离物。共鉴定出 41 个物种，隶属于子囊菌界的 17 个属和基生菌界的 2 个属，其中曲霉属在洞穴中最常见（13 个类群）。在沉积物中发现了 24 个菌种（16 个独家），在空气中发现了 25 个菌种（17 个独家）。在这项研究中，我们从沉积物和空气中获得的真菌分离物中引入了虫草科的一个新属（Pseudolecanicillium gen.nov.）和六个新种（占已鉴定类群总数的 14%）：此外，我们还建立了一份巴西洞穴真菌分类群清单。我们的研究结果凸显了巴西洞穴对国家和全球真菌多样性的贡献。引用：Alves VCS, Lira RA, Lima JMS, Barbosa RN, Bento DM, Barbier E, Bernard E, Souza-Motta CM, Bezerra JDP (2022)。揭开热带洞穴中真菌的神秘面纱：丰富的真菌以及一个新属和六个新种的描述。Fungal Systematics and Evolution 10: 139-167. doi: 10.3114/fuse.2022.10.06.
Unravelling the fungal darkness in a tropical cave: richness and the description of one new genus and six new species.
Caves are special environments that harbour an incredible diversity of life, including fungal species. Brazilian caves have been demonstrated to be biodiversity hotspots for known and unknown fungal species. We investigated the richness of culturable fungi in a tropical cave in Brazil by isolating these microorganisms from the sediment and air. The fungal abundance of colony-forming units (CFUs) was 3 178 in sediment and 526 in air. We used morphological features and phylogenetic analyses of actin (actA), calmodulin (cmdA), internal transcribed spacer regions and intervening 5.8S rRNA (ITS), large subunit (LSU) rDNA, RNA polymerase II second largest subunit (rpb2), translation elongation factor 1-alpha (tef1), and β-tubulin (tub2) genes to identify these isolates. Forty-one species belonging to 17 genera of Ascomycota and two of Basidiomycota were identified, and the genus Aspergillus was most commonly observed in the cave (13 taxa). Twenty-four species were found in sediment (16 exclusives) and 25 species were found in air (17 exclusives). In this study, we introduced a new genus (Pseudolecanicillium gen. nov.) in the family Cordycipitaceae and six new species (14 % of the total taxa identified) of fungal isolates obtained from sediment and air: Aspergillus lebretii sp. nov., Malbranchea cavernosa sp. nov., Pseudohumicola cecavii sp. nov., Pseudolecanicillium caatingaense sp. nov., Talaromyces cavernicola sp. nov., and Tritirachium brasiliensesp. nov. In addition, we built a checklist of the fungal taxa reported from Brazilian caves. Our results highlight the contribution of Brazilian caves to the estimation of national and global fungal diversity. Citation: Alves VCS, Lira RA, Lima JMS, Barbosa RN, Bento DM, Barbier E, Bernard E, Souza-Motta CM, Bezerra JDP (2022). Unravelling the fungal darkness in a tropical cave: richness and the description of one new genus and six new species. Fungal Systematics and Evolution10: 139-167. doi: 10.3114/fuse.2022.10.06.
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