牛围排卵期卵泡液的生化变化及其与卵母细胞最终成熟的关系

Noof Abdulrahman Alrabiah, Constantine A Simintiras, Alexander C O Evans, Patrick Lonergan, Trudee Fair
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引用次数: 0

摘要

卵泡液(FF)是血管渗出物、颗粒细胞和鳞状细胞分泌物的产物,是支持卵母细胞生长和成熟的环境,在决定卵母细胞质量方面起着核心作用。因此,不理想的FF成分可能会影响卵母细胞的成熟、受精或胚胎发育。迄今为止,对牛 FF 成分的研究仍然不足。为了解决这个问题,我们全面描述了牛卵母细胞减数分裂成熟期的 FF 代谢组学组成。更具体地说,我们采用高通量非靶向超高效液相色谱串联质谱分析了排卵前(-24 小时)和围排卵期(-2 小时)卵泡的FF。共鉴定出 634 种代谢物,包括:脂类(37.1%)、氨基酸(30.0%)、异种生物(11.5%)、核苷酸(6.8%)、碳水化合物(4.4%)、辅助因子和维生素(4.4%)、肽(3.6%)和能量底物(2.1%)。67种代谢物的浓度受卵泡发育阶段的显著影响,33.3%(n=21)的代谢物浓度降低(P≤0.05),平均降低9.0倍,而46种代谢物浓度升高(P≤0.05),平均升高1.7倍。单个代谢物浓度下降最明显的是次黄嘌呤(98.9 倍)、黄嘌呤(65.7 倍)、17β-雌二醇(12.4 倍)和肌苷(4.6 倍)。相比之下,增加最多的是视黄醛(4.9 倍)、1-甲基-5-咪唑乙酸酯(2.7 倍)和异戊酰肉碱(2.7 倍)。这种对牛 FF 时间动态的全球代谢组学分析为了解支持卵母细胞成熟和促进排卵的环境提供了新的信息,有可能提高体内和体外卵母细胞的质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biochemical alterations in the follicular fluid of bovine peri-ovulatory follicles and association with final oocyte maturation.

Biochemical alterations in the follicular fluid of bovine peri-ovulatory follicles and association with final oocyte maturation.

Biochemical alterations in the follicular fluid of bovine peri-ovulatory follicles and association with final oocyte maturation.

Biochemical alterations in the follicular fluid of bovine peri-ovulatory follicles and association with final oocyte maturation.

Follicular fluid (FF), a product of vascular transudate and granulosa and thecal cell secretions, is the milieu that has evolved to support oocyte growth and maturation which plays a central role in oocyte quality determination. Therefore, a suboptimal FF composition may be reflected in compromised oocyte progression through maturation, fertilization or embryo development. To date, the composition of bovine FF remains understudied. To address this, we comprehensively characterized the metabolomic constituency of bovine FF in the period during which the oocyte undergoes meiotic maturation. More specifically, FF from pre (-24 h) and peri (-2 h) -ovulatory follicles was profiled by high-throughput untargeted ultra-high-performance liquid chromatography tandem mass spectroscopy. A total of 634 metabolites were identified, comprising: lipids (37.1%), amino acids (30.0%), xenobiotics (11.5%), nucleotides (6.8%), carbohydrates (4.4%), cofactors and vitamins (4.4%), peptides (3.6%) and energy substrates (2.1%). The concentrations of 67 metabolites were significantly affected by stage of follicle development, 33.3% (n=21) were reduced (P≤0.05) by a mean of 9.0-fold, whereas 46 were elevated (P≤0.05) by a mean of 1.7-fold in peri vs. pre -ovulatory FF. The most pronounced individual metabolite concentration decreases were hypoxanthine (98.9-fold), xanthine (65.7-fold), 17β-oestradiol (12.4-fold), and inosine (4.6-fold). In contrast, the greatest increases were in retinal (4.9-fold), 1-methyl-5-imidazoleacetate (2.7-fold), and isovalerylcarnitine (2.7-fold). This global metabolomic analysis of bovine FF temporal dynamics provides new information for understanding the environment supporting oocyte maturation and facilitating ovulation, that has the potential for improving oocyte quality both in vivo and in vitro.

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