Louis Garneau, Pascale B Beauregard, Sébastien Roy
{"title":"根瘤中的邻居:桤木根瘤内生菌Frankia sp. ACN10a与非Frankia根瘤内生菌的相互作用。","authors":"Louis Garneau, Pascale B Beauregard, Sébastien Roy","doi":"10.1139/cjm-2022-0074","DOIUrl":null,"url":null,"abstract":"<p><p>In the present study, we report the in vitro interactions between <i>Frankia</i> sp. ACN10a and non-<i>Frankia</i> nodular endophytes (NFNE) isolated from alder. The supernatant of NFNE grown in nitrogen-replete medium had neutral or negative effects on <i>Frankia</i> growth; none had a stimulatory effect. Inhibitory effects were observed for supernatants of some NFNE, notably <i>Micromonospora, Pseudomonas, Serratia</i> and <i>Stenotrophomonas</i> isolates. However, some NFNE-<i>Frankia</i> coculture supernatants could stimulate <i>Frankia</i> growth when used as a culture medium supplement. This was observed for supernatants of <i>Frankia</i> cocultured with <i>Microvirga</i> and S<i>treptomyces</i> isolates. In nitrogen-limited conditions, cocultures of <i>Frankia</i> with some NFNE, including some rhizobia and <i>Cytobacillus,</i> resulted in higher total biomass than <i>Frankia</i>-only cultures, suggesting cooperation, while other NFNE were strongly antagonistic. Microscopic observation of cocultures also revealed compromised <i>Frankia</i> membrane integrity, and some differentiation into stress resistance-associated morphotypes such as sporangia and reproductive torulose hyphae (RTH). Furthermore, the coculture of <i>Frankia</i> with <i>Serratia</i> sp. isolates resulted in higher concentrations of the auxinic plant hormone indole-3-acetic acid and related indolic compounds in the culture supernatant. This study sheds new light on the breadth of microbial interactions that occur amongst bacteria that inhabit the understudied ecological niche of the alder nodule.</p>","PeriodicalId":9381,"journal":{"name":"Canadian journal of microbiology","volume":"69 2","pages":"88-102"},"PeriodicalIF":1.8000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Neighbours in nodules: the interactions between <i>Frankia</i> sp. ACN10a and non-<i>Frankia</i> nodular endophytes of alder.\",\"authors\":\"Louis Garneau, Pascale B Beauregard, Sébastien Roy\",\"doi\":\"10.1139/cjm-2022-0074\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In the present study, we report the in vitro interactions between <i>Frankia</i> sp. ACN10a and non-<i>Frankia</i> nodular endophytes (NFNE) isolated from alder. The supernatant of NFNE grown in nitrogen-replete medium had neutral or negative effects on <i>Frankia</i> growth; none had a stimulatory effect. Inhibitory effects were observed for supernatants of some NFNE, notably <i>Micromonospora, Pseudomonas, Serratia</i> and <i>Stenotrophomonas</i> isolates. However, some NFNE-<i>Frankia</i> coculture supernatants could stimulate <i>Frankia</i> growth when used as a culture medium supplement. This was observed for supernatants of <i>Frankia</i> cocultured with <i>Microvirga</i> and S<i>treptomyces</i> isolates. In nitrogen-limited conditions, cocultures of <i>Frankia</i> with some NFNE, including some rhizobia and <i>Cytobacillus,</i> resulted in higher total biomass than <i>Frankia</i>-only cultures, suggesting cooperation, while other NFNE were strongly antagonistic. Microscopic observation of cocultures also revealed compromised <i>Frankia</i> membrane integrity, and some differentiation into stress resistance-associated morphotypes such as sporangia and reproductive torulose hyphae (RTH). Furthermore, the coculture of <i>Frankia</i> with <i>Serratia</i> sp. isolates resulted in higher concentrations of the auxinic plant hormone indole-3-acetic acid and related indolic compounds in the culture supernatant. This study sheds new light on the breadth of microbial interactions that occur amongst bacteria that inhabit the understudied ecological niche of the alder nodule.</p>\",\"PeriodicalId\":9381,\"journal\":{\"name\":\"Canadian journal of microbiology\",\"volume\":\"69 2\",\"pages\":\"88-102\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canadian journal of microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1139/cjm-2022-0074\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian journal of microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1139/cjm-2022-0074","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Neighbours in nodules: the interactions between Frankia sp. ACN10a and non-Frankia nodular endophytes of alder.
In the present study, we report the in vitro interactions between Frankia sp. ACN10a and non-Frankia nodular endophytes (NFNE) isolated from alder. The supernatant of NFNE grown in nitrogen-replete medium had neutral or negative effects on Frankia growth; none had a stimulatory effect. Inhibitory effects were observed for supernatants of some NFNE, notably Micromonospora, Pseudomonas, Serratia and Stenotrophomonas isolates. However, some NFNE-Frankia coculture supernatants could stimulate Frankia growth when used as a culture medium supplement. This was observed for supernatants of Frankia cocultured with Microvirga and Streptomyces isolates. In nitrogen-limited conditions, cocultures of Frankia with some NFNE, including some rhizobia and Cytobacillus, resulted in higher total biomass than Frankia-only cultures, suggesting cooperation, while other NFNE were strongly antagonistic. Microscopic observation of cocultures also revealed compromised Frankia membrane integrity, and some differentiation into stress resistance-associated morphotypes such as sporangia and reproductive torulose hyphae (RTH). Furthermore, the coculture of Frankia with Serratia sp. isolates resulted in higher concentrations of the auxinic plant hormone indole-3-acetic acid and related indolic compounds in the culture supernatant. This study sheds new light on the breadth of microbial interactions that occur amongst bacteria that inhabit the understudied ecological niche of the alder nodule.
期刊介绍:
Published since 1954, the Canadian Journal of Microbiology is a monthly journal that contains new research in the field of microbiology, including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.