用于免疫组织化学的名义过期抗体的验证。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Anthony F Henwood
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引用次数: 0

摘要

韦斯特米德儿童医院的组织病理学部门在其免疫组织化学小组中有114种抗体;其中64种是作为浓缩物购买的,通常在实验室收到后1-2年到期。在过期后更换这些抗体将需要每年大约40,000美元。据报道,这些试剂在过期后继续使用可能是可行的。我使用ippassport质量管理系统跟踪抗体有效期,并验证这些试剂的延长适用性。iPassport是一款基于网络的质量管理软件,可帮助医学实验室进行文件控制和质量管理。对ippassport自2015年开始使用以来的记录的审查表明,到目前为止,没有失败的验证,到期后的平均寿命为6年;8个抗体超过6年。一些抗体的寿命特别长,包括因子8(21年)、因子13a(19年)和上皮膜抗原(17年)。选择要丢弃的抗体应该基于性能而不是有效期。ippassport质量管理体系能够对名义过期的抗体进行永久记录和定期验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of nominally expired antibodies for immunohistochemistry.

The Histopathology Department at the Children's Hospital at Westmead has 114 antibodies in its Immunohistochemistry panel; 64 of these are purchased as concentrates and usually have expiration dates 1-2 years after receipt by the laboratory. To replace these antibodies after expiration would require approximately $40,000/year. It has been reported that continued use of these reagents beyond their expiration dates may be feasible. I used the iPassport quality management system to track antibody expiration dates and verified extended fit-for-purpose for these reagents. iPassport is web-based quality management software that assists medical laboratories with document control and quality management. Review of the records since the inception of iPassport in 2015 indicates no failed verifications and to date, the average life after expiration is 6 years; eight antibodies have exceeded 6 years. Some antibodies with exceptionally extended lifespans include factor 8 (21 years), factor 13a (19 years) and epithelial membrane antigen (17 years). Selecting antibodies to be discarded should be based on performance rather than expiration date alone. The iPassport quality management system has enabled permanent recording and periodic validation of nominally expired antibodies.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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