蛋白质印迹（免疫印迹）：历史、理论、用途、方案和问题。

IF 2.2 4区工程技术 Q3 BIOCHEMICAL RESEARCH METHODS

BioTechniques Pub Date : 2023-09-01 Epub Date: 2023-03-27 DOI:10.2144/btn-2022-0034

Rasheed Sule, Gabriela Rivera, Aldrin V Gomes

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引用次数: 2

摘要

蛋白质印迹（免疫印迹）是一种强大且常用的技术，能够从从细胞或组织中提取的蛋白质的复杂混合物中检测或半定量单个蛋白质。介绍了蛋白质印迹的起源、蛋白质印迹技术背后的理论、综合方案和蛋白质印迹的用途。重点讨论了蛋白质印迹领域中鲜为人知的重大问题以及常见问题的疑难解答。这项工作为新的蛋白质印迹研究人员和那些对更好地理解该技术或获得更好结果感兴趣的人提供了全面的引物和指南。

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Western blotting (immunoblotting): history, theory, uses, protocol and problems.

Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues. The history surrounding the origin of western blotting, the theory behind the western blotting technique, a comprehensive protocol and the uses of western blotting are presented. Lesser known and significant problems in the western blotting field and troubleshooting of common problems are highlighted and discussed. This work is a comprehensive primer and guide for new western blotting researchers and those interested in a better understanding of the technique or getting better results.

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来源期刊

BioTechniques 工程技术-生化研究方法

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

3.3 months

期刊介绍： BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.