Cheol Park, Hyesook Lee, Sung Ok Kim, Eun-Woo Lee, Hyun-Tai Lee, Hyun Ju Kwon, Byung Woo Kim, Gi-Young Kim, Mi Ryeo Kim, Yung Hyun Choi
{"title":"森罗木麻通过 Nrf2 介导的 HO-1 激活，对氧化应激诱导的骨骼 L6 肌母细胞细胞损伤具有预防作用。","authors":"Cheol Park, Hyesook Lee, Sung Ok Kim, Eun-Woo Lee, Hyun-Tai Lee, Hyun Ju Kwon, Byung Woo Kim, Gi-Young Kim, Mi Ryeo Kim, Yung Hyun Choi","doi":"10.1007/s43188-022-00141-5","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of the present study is to investigate the preventive effect of water extract of <i>Mori Ramulus</i> (MRWE) on oxidative stress-mediated cellular damages in rat skeletal L6 myoblasts. Our results demonstrated that MRWE pretreatment markedly improved cell survival and suppressed cell cycle arrest at the G2/M phase and apoptosis in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-treated L6 cells. H<sub>2</sub>O<sub>2</sub>-triggered DNA damage was also notably reduced by MRWE, which since it was correlated with protection of reactive oxygen species (ROS) production. Additionally, H<sub>2</sub>O<sub>2</sub> stimulated cytosolic release of cytochrome <i>c</i> and up-regulation of Bax/Bcl-2 ratio, whereas MRWE suppressed these changes following by H<sub>2</sub>O<sub>2</sub>. Moreover, MRWE inhibited the cleavage of poly(ADP-ribose) polymerase as well as the activity of caspase-3 by H<sub>2</sub>O<sub>2</sub>. Furthermore, MRWE enhanced H<sub>2</sub>O<sub>2</sub>-mediated expression of nuclear factor erythroid 2-associated factor 2 (Nrf2) and its representative downstream enzyme, heme oxygenase-1 (HO-1). However, the protective effects of MRWE on H<sub>2</sub>O<sub>2</sub>-induced ROS production, cell cycle arrest and apoptosis were significantly attenuated by HO-1 inhibitor. In conclusion, our present results suggests that MRWE could protect L6 myoblasts from H<sub>2</sub>O<sub>2</sub>-induced cellular injury by inhibiting ROS generation along with Nrf2-mediated activation of HO-1, indicating this finding may expand the scope of application of <i>Mori Ramulus</i> in medicine.</p>","PeriodicalId":23181,"journal":{"name":"Toxicological Research","volume":"39 1","pages":"25-36"},"PeriodicalIF":1.6000,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839907/pdf/","citationCount":"3","resultStr":"{\"title\":\"The preventive effect of <i>Mori Ramulus</i> on oxidative stress-induced cellular damage in skeletal L6 myoblasts through Nrf2-mediated activation of HO-1.\",\"authors\":\"Cheol Park, Hyesook Lee, Sung Ok Kim, Eun-Woo Lee, Hyun-Tai Lee, Hyun Ju Kwon, Byung Woo Kim, Gi-Young Kim, Mi Ryeo Kim, Yung Hyun Choi\",\"doi\":\"10.1007/s43188-022-00141-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of the present study is to investigate the preventive effect of water extract of <i>Mori Ramulus</i> (MRWE) on oxidative stress-mediated cellular damages in rat skeletal L6 myoblasts. Our results demonstrated that MRWE pretreatment markedly improved cell survival and suppressed cell cycle arrest at the G2/M phase and apoptosis in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-treated L6 cells. H<sub>2</sub>O<sub>2</sub>-triggered DNA damage was also notably reduced by MRWE, which since it was correlated with protection of reactive oxygen species (ROS) production. Additionally, H<sub>2</sub>O<sub>2</sub> stimulated cytosolic release of cytochrome <i>c</i> and up-regulation of Bax/Bcl-2 ratio, whereas MRWE suppressed these changes following by H<sub>2</sub>O<sub>2</sub>. Moreover, MRWE inhibited the cleavage of poly(ADP-ribose) polymerase as well as the activity of caspase-3 by H<sub>2</sub>O<sub>2</sub>. Furthermore, MRWE enhanced H<sub>2</sub>O<sub>2</sub>-mediated expression of nuclear factor erythroid 2-associated factor 2 (Nrf2) and its representative downstream enzyme, heme oxygenase-1 (HO-1). However, the protective effects of MRWE on H<sub>2</sub>O<sub>2</sub>-induced ROS production, cell cycle arrest and apoptosis were significantly attenuated by HO-1 inhibitor. In conclusion, our present results suggests that MRWE could protect L6 myoblasts from H<sub>2</sub>O<sub>2</sub>-induced cellular injury by inhibiting ROS generation along with Nrf2-mediated activation of HO-1, indicating this finding may expand the scope of application of <i>Mori Ramulus</i> in medicine.</p>\",\"PeriodicalId\":23181,\"journal\":{\"name\":\"Toxicological Research\",\"volume\":\"39 1\",\"pages\":\"25-36\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2022-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839907/pdf/\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxicological Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s43188-022-00141-5\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s43188-022-00141-5","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}
The preventive effect of Mori Ramulus on oxidative stress-induced cellular damage in skeletal L6 myoblasts through Nrf2-mediated activation of HO-1.
The aim of the present study is to investigate the preventive effect of water extract of Mori Ramulus (MRWE) on oxidative stress-mediated cellular damages in rat skeletal L6 myoblasts. Our results demonstrated that MRWE pretreatment markedly improved cell survival and suppressed cell cycle arrest at the G2/M phase and apoptosis in hydrogen peroxide (H2O2)-treated L6 cells. H2O2-triggered DNA damage was also notably reduced by MRWE, which since it was correlated with protection of reactive oxygen species (ROS) production. Additionally, H2O2 stimulated cytosolic release of cytochrome c and up-regulation of Bax/Bcl-2 ratio, whereas MRWE suppressed these changes following by H2O2. Moreover, MRWE inhibited the cleavage of poly(ADP-ribose) polymerase as well as the activity of caspase-3 by H2O2. Furthermore, MRWE enhanced H2O2-mediated expression of nuclear factor erythroid 2-associated factor 2 (Nrf2) and its representative downstream enzyme, heme oxygenase-1 (HO-1). However, the protective effects of MRWE on H2O2-induced ROS production, cell cycle arrest and apoptosis were significantly attenuated by HO-1 inhibitor. In conclusion, our present results suggests that MRWE could protect L6 myoblasts from H2O2-induced cellular injury by inhibiting ROS generation along with Nrf2-mediated activation of HO-1, indicating this finding may expand the scope of application of Mori Ramulus in medicine.
期刊介绍：
Toxicological Research is the official journal of the Korean Society of Toxicology. The journal covers all areas of Toxicological Research of chemicals, drugs and environmental agents affecting human and animals, which in turn impact public health. The journal’s mission is to disseminate scientific and technical information on diverse areas of toxicological research. Contributions by toxicologists, molecular biologists, geneticists, biochemists, pharmacologists, clinical researchers and epidemiologists with a global view on public health through toxicological research are welcome. Emphasis will be given to articles providing an understanding of the toxicological mechanisms affecting animal, human and public health. In the case of research articles using natural extracts, detailed information with respect to the origin, extraction method, chemical profiles, and characterization of standard compounds to ensure the reproducible pharmacological activity should be provided.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
