枣河-渭河低氧带抗生素耐药基因的时间动态:由氧和细菌群落驱动

IF 2.4 4区 环境科学与生态学 Q2 ECOLOGY
Ruyi Feng, Lei Duan, Siqi Shen, Yan Cheng, Yanhua Wang, Wenke Wang, Shengke Yang
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引用次数: 3

摘要

抗生素耐药基因(ARGs)在潜流带(HZ)的广泛传播由于其潜在的危害性已成为一个新兴的环境问题。本研究设置了三种不同的氧处理系统,研究氧变化对HZ中ARGs丰度的影响。此外,还研究了温度和盐度对好氧和厌氧系统下ARGs的影响。分析了沉积物样品的细菌群落组成及其与ARGs的关系。采用变异分配分析(VPA)和结构方程模型(SEM)分析了影响ARGs驱动因素的解释比和因果关系。厌氧系统中ARGs和移动遗传元件(mobile genetic elements, MGEs)的相对丰度显著增加,高于好氧系统和好氧-厌氧相互作用系统。盐度和温度的实验也进一步证实了这一结果。沉积物中影响四环素类和磺胺类ARGs的细菌群落较多,这些宿主细菌主要集中在变形菌门、厚壁菌门和拟杆菌门。VPA和SEM进一步显示,ARGs的丰度主要受细菌群落和氧条件变化的影响,MGEs的水平基因转移(HGT)对ARGs的传播也有积极的影响。这些发现表明,HZ中复杂的氧气条件改变了细菌群落,促进了mges介导的水平转移,这些共同导致了ARGs的传播。该研究对制定有效的策略以减少ARGs在地下环境中的传播具有参考价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Temporal dynamic of antibiotic resistance genes in the Zaohe-Weihe hyporheic zone: driven by oxygen and bacterial community.

The widespread spread of antibiotic resistance genes (ARGs) in hyporheic zone (HZ) has become an emerging environmental problem due to their potentially harmful nature. In this research, three different oxygen treatment systems were set up to study the effects of oxygen changes on the abundance of ARGs in the HZ. In addition, the effects of temperature and salinity on ARGs were investigated under aerobic and anaerobic systems, respectively. The bacterial community composition of sediment samples and the relationship with ARGs were analyzed. The explanation ratio and causality of the driving factors affecting ARGs were analyzed using variation partitioning analysis (VPA) and structural equation model (SEM). The relative abundance of ARGs and mobile genetic elements (MGEs) in the anaerobic system increased significantly, which was higher than that in the aerobic system and the aerobic-anaerobic interaction system. The experiment of salinity and temperature also further proved this result. There were many bacterial communities that affected tetracycline and sulfonamide ARGs in sediments, and these host bacteria are mainly concentrated in Proteobacteria, Firmicutes and Bacteroidetes. VPA and SEM further revealed that the abundance of ARGs was mainly influenced by changes in bacterial communities and oxygen conditions, and horizontal gene transfer (HGT) of MGEs also had a positive effect on the spread of ARGs. Those findings suggest that complex oxygen conditions in the HZ alter bacterial communities and promote MGEs-mediated horizontal transfer, which together lead to the spread of ARGs. This study has value as a reference for formulating effective strategies to minimize the propagation of ARGs in underground environment.

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来源期刊
Ecotoxicology
Ecotoxicology 环境科学-毒理学
CiteScore
5.30
自引率
3.70%
发文量
107
审稿时长
4.7 months
期刊介绍: Ecotoxicology is an international journal devoted to the publication of fundamental research on the effects of toxic chemicals on populations, communities and terrestrial, freshwater and marine ecosystems. It aims to elucidate mechanisms and processes whereby chemicals exert their effects on ecosystems and the impact caused at the population or community level. The journal is not biased with respect to taxon or biome, and papers that indicate possible new approaches to regulation and control of toxic chemicals and those aiding in formulating ways of conserving threatened species are particularly welcome. Studies on individuals should demonstrate linkage to population effects in clear and quantitative ways. Laboratory studies must show a clear linkage to specific field situations. The journal includes not only original research papers but technical notes and review articles, both invited and submitted. A strong, broadly based editorial board ensures as wide an international coverage as possible.
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