1-烷基-1-甲基溴化哌啶对真菌质膜脂质及肺表面活性剂的影响

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Katarzyna Dopierała, Anna Syguda, Marta Wojcieszak, Katarzyna Materna
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引用次数: 1

摘要

本研究旨在探讨1-烷基-1-甲基哌啶溴作为杀菌剂的潜力,并评估其在大气中传播时对人体呼吸系统的影响。我们研究了一系列两亲性的1-烷基-1-甲基哌啶溴化物([MePipCn][Br])存在时膜脂和模型膜的行为,不同的烷基链长(n = 4−18)。实验采用Langmuir单层技术,分别以肺表面活性剂和真菌质膜的主要成分1,2-双棕榈酰- n-甘油-3-磷脂胆碱(DPPC)和麦角甾醇(ERG)及其与磷脂和甾醇的混合物为主要成分。选择混合物作为靶生物和非靶生物的代表。在亚相中有[MePipCn][Br]存在的情况下,通过压缩单层得到表面压力-面积等温线。对结果进行了面积膨胀/收缩和可压缩性分析。研究了所研究的有机盐类的表面活性。此外,单层膜沉积在固体表面,并使用原子力显微镜研究其形貌。本研究表明,所研究的化合物可以有效地破坏真菌质膜的稳定性。同时,我们证明了1-烷基-1-甲基溴化哌啶对肺表面活性物质层的显著影响。[MePipCn][Br]与模型膜的相互作用取决于有机盐的浓度和烷基链长度。同时也揭示了接触时间的关键作用。研究结果可为合理开发针对植物真菌感染的新型农化产品提供参考。此外,我们的研究表明了杀菌剂在环境中传播时进行适当的安全管理的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of 1-alkyl-1-methylpiperidinium bromides on lipids of fungal plasma membrane and lung surfactant

Effect of 1-alkyl-1-methylpiperidinium bromides on lipids of fungal plasma membrane and lung surfactant

This study aimed to investigate the potential of 1-alkyl-1-methylpiperidinium bromides as fungicides and evaluate their impact on the human respiratory system when spread in the atmosphere. We investigated the behavior of membrane lipids and model membranes in the presence of a series of amphiphilic 1-alkyl-1-methylpiperidinium bromides ([MePipCn][Br]), differing in the alkyl chain length (n = 4 − 18). The experiments were performed with the Langmuir monolayer technique using 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and ergosterol (ERG)–the main components of lung surfactant and fungal plasma membrane, respectively and their mixtures with phospholipids and sterols. The mixtures were chosen as the representatives of target and non−target organisms. The surface pressure−area isotherms were obtained by compressing monolayers in the presence of [MePipCn][Br] in the subphase. The results were analyzed in terms of area expansion/contraction and compressibility. The surface activity of the studied organic salts was also studied. In addition, the monolayers were deposited on a solid surface and their topography was investigated using atomic force microscopy. This research implies that the studied compounds may destabilize efficiently the fungal plasma membrane. At the same time we demonstrated the significant impact of 1-alkyl-1-methylpiperidinium bromides on the lung surfactant layer. The interaction between [MePipCn][Br] and model membranes depends on the concentration and alkyl chain length of organic salt. The key role of contact time has been also revealed. The results may be helpful in the reasonable development of new agrochemical products aiming at the treatment of fungal infections in plants. In addition, our study indicates the significance of proper safety management while spreading the fungicides in the environment.

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来源期刊
Chemistry and Physics of Lipids
Chemistry and Physics of Lipids 生物-生化与分子生物学
CiteScore
7.60
自引率
2.90%
发文量
50
审稿时长
40 days
期刊介绍: Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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