盐酸盐受体GluK1和GluK2调节突触形态的差异。

IF 1.6 4区 医学 Q4 NEUROSCIENCES
Synapse Pub Date : 2023-01-01 DOI:10.1002/syn.22255
Gui-Fang Duan, Xiao-Hui Tang, Min Jia, Dan Wu, Yun Stone Shi
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引用次数: 1

摘要

树突棘形态的调控是发育过程中神经元网络细化和神经传递调节的重要方面。先前的研究表明,谷氨酸传递在突触发育中起着核心作用。AMPA受体和NMDA受体以活动依赖的方式调节脊柱形态。然而,Kainate受体(KARs)是否以及如何调节突触发育仍然知之甚少。在这项研究中,我们发现GluK1和GluK2可能在突触发育中发挥不同的作用。在原代培养的海马神经元中,我们发现钙渗透性GluK2(Q)受体变体的过表达比钙不渗透性GluK2(R)变体的过表达或EGFP转染的对照神经元增加了脊柱长度和脊柱头部面积,这表明Q/R编辑可能在GluK2调节突触发育中发挥作用。有趣的是,转染GluK1(Q)的神经元脊柱长度和脊柱头面积减少,而树突棘密度增加,这表明GluK1(Q)和GluK2(Q)对突触发育的影响不同。在GluK2和GluK1之间交换关键结构域证明了n端结构域(NTD)是GluK1和GluK2不同作用的原因。综上所述,Kainate受体GluK1和GluK2在调节脊柱形态和发育中具有不同的作用,这一过程可能依赖于NTD。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Kainate receptors GluK1 and GluK2 differentially regulate synapse morphology.

Kainate receptors GluK1 and GluK2 differentially regulate synapse morphology.

The regulation of dendritic spine morphology is a critical aspect of neuronal network refinement during development and modulation of neurotransmission. Previous studies revealed that glutamatergic transmission plays a central role in synapse development. AMPA receptors and NMDA receptors regulate spine morphology in an activity dependent manner. However, whether and how Kainate receptors (KARs) regulate synapse development remains poorly understood. In this study, we found that GluK1 and GluK2 may play distinct roles in synapse development. In primary cultured hippocampal neurons, we found overexpression of the calcium-permeable GluK2(Q) receptor variant increased spine length and spine head area compared to overexpression of the calcium-impermeable GluK2(R) variant or EGFP transfected, control neurons, indicating that Q/R editing may play a role in GluK2 regulation of synapse development. Intriguingly, neurons transfected with GluK1(Q) showed decreased spine length and spine head area, while the density of dendritic spines was increased, suggesting that GluK1(Q) and GluK2(Q) have different effects on synaptic development. Swapping the critical domains between GluK2 and GluK1 demonstrated the N-terminal domain (NTD) is responsible for the different effects of GluK1 and GluK2. In conclusion, Kainate receptors GluK1 and GluK2 have distinct roles in regulating spine morphology and development, a process likely relying on the NTD.

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来源期刊
Synapse
Synapse 医学-神经科学
CiteScore
3.80
自引率
0.00%
发文量
38
审稿时长
4-8 weeks
期刊介绍: SYNAPSE publishes articles concerned with all aspects of synaptic structure and function. This includes neurotransmitters, neuropeptides, neuromodulators, receptors, gap junctions, metabolism, plasticity, circuitry, mathematical modeling, ion channels, patch recording, single unit recording, development, behavior, pathology, toxicology, etc.
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