伽玛辐射体外灭活SARS-CoV-2的研究

IF 0.5 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Applied Biosafety Pub Date : 2020-09-01 DOI:10.1177/1535676020934242

Anders Leung, Kaylie Tran, Jonathan Audet, Sherisse Lavineway, Nathalie Bastien, Jay Krishnan

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引用次数: 20

摘要

简介:严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)被列为危险组3;这种活病毒的繁殖工作应在生物安全3级(BSL-3)实验室进行。然而，灭活病毒可以在生物安全二级实验室安全地处理。射线照射是用来灭活包括病毒在内的多种病原体的方法之一。目的:确定灭活SARS-CoV-2所需的辐射剂量及其对随后的聚合酶链反应(PCR)试验的影响(如果有的话)。方法:将等量的SARS-CoV-2病毒培养物进行增加剂量的伽马辐射，以确定灭活病毒所需的适当剂量。将辐照样品的实时定量聚合酶链反应(RT-qPCR)数据与未辐照样品的实时定量聚合酶链反应(RT-qPCR)数据进行比较，以评估伽马辐射对PCR检测的影响。结果:1 Mrad的辐射剂量可完全灭活106.5 TCID50/ml的SARS-CoV-2。γ辐射对PCR敏感性的影响呈负相关且剂量依赖性，最高可达0.5 Mrad，此后没有进一步降低。结论:伽玛辐照可作为一种可靠的灭活SARS-CoV-2的方法，对后续PCR检测影响最小。

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In Vitro Inactivation of SARS-CoV-2 Using Gamma Radiation.

Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is classified as a Risk Group 3 pathogen; propagative work with this live virus should be conducted in biosafety level-3 (BSL-3) laboratories. However, inactivated virus can be safely handled in BSL-2 laboratories. Gamma irradiation is one of the methods used to inactivate a variety of pathogens including viruses.

Objective: To determine the radiation dose required to inactivate SARS-CoV-2 and its effect, if any, on subsequent polymerase chain reaction (PCR) assay.

Methods: Aliquots of SARS-CoV-2 virus culture were subjected to increasing doses of gamma radiation to determine the proper dose required to inactivate the virus. Real-time quantitative polymerase chain reaction (RT-qPCR) data from irradiated samples was compared with that of the non-irradiated samples to assess the effect of gamma radiation on PCR assay.

Results: A radiation dose of 1 Mrad was required to completely inactivate 10^6.5 TCID₅₀/ml of SARS-CoV-2. The influence of gamma radiation on PCR sensitivity was inversely related and dose-dependent up to 0.5 Mrad with no further reduction thereafter.

Conclusion: Gamma irradiation can be used as a reliable method to inactivate SARS-CoV-2 with minimal effect on subsequent PCR assay.

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来源期刊

Applied Biosafety Environmental Science-Management, Monitoring, Policy and Law

CiteScore

2.50

自引率

13.30%

发文量

期刊介绍： Applied Biosafety (APB), sponsored by ABSA International, is a peer-reviewed, scientific journal committed to promoting global biosafety awareness and best practices to prevent occupational exposures and adverse environmental impacts related to biohazardous releases. APB provides a forum for exchanging sound biosafety and biosecurity initiatives by publishing original articles, review articles, letters to the editors, commentaries, and brief reviews. APB informs scientists, safety professionals, policymakers, engineers, architects, and governmental organizations. The journal is committed to publishing on topics significant in well-resourced countries as well as information relevant to underserved regions, engaging and cultivating the development of biosafety professionals globally.