INPP5A/HLA-G1/IL-10/MMP-21轴在食管鳞状细胞癌进展中的作用

Q2 Biochemistry, Genetics and Molecular Biology
Sima Ardalan Khales, Azadeh Aarabi, Mohammad Reza Abbaszadegan, Mohammad Mahdi Forghanifard
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引用次数: 1

摘要

背景:I型肌醇多磷酸-5磷酸酶A (INPP5A)参与不同的细胞事件,包括细胞增殖。由于INPP5A、HLAG1、IL-10和基质金属蛋白酶(MMP)-21基因在食管鳞状细胞癌(ESCC)的肿瘤发生中起着重要作用,我们在本研究中旨在阐明这些基因可能的相互作用,并探索这些化学物质作为ESCC疾病诊断的预测标志物的潜力。方法:采用相对实时荧光定量PCR方法,对56例escc与边缘正常组织进行INPP5A、HLAG-1、IL-10、MMP-21基因表达分析。在escc中对INPP5A进行免疫组化染色。应用ROC曲线分析和AUC评价候选基因的诊断能力。结果:与正常组织相比,HLA-G1、MMP-21和IL-10分别在近23.2%、62.5%和53.5%的escc中检测到高表达,而INPP5A在19.6%的escc中检测到低表达,所有被测基因之间均存在显著相关性。INPP5A蛋白在ESCC组织中的表达水平明显低于非肿瘤食管组织(p = 0.001)。有趣的是,INPP5A/HLA-G1、INPP5A/MMP-21、INPP5A/IL-10、HLA-G1/MMP-21、HLA-G1/IL-10和MMP-21/IL-10的同时表达与多个临床病理变量显著相关。INPP5A、HLA-G1、MMP-21和IL-10是区分肿瘤/非肿瘤组最合适的候选,因为所有组合的总auc(>60%)。结论:我们的研究结果代表了一个包含INPP5A/HLAG-1/IL-10/MMP-21标志物的新调控轴在ESCC的发展过程中,并可能为INPP5A/HLAG-1/IL-10/MMP-21调控网络介导的免疫逃避机制提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

INPP5A/HLA-G1/IL-10/MMP-21 Axis in Progression of Esophageal Squamous Cell Carcinoma

INPP5A/HLA-G1/IL-10/MMP-21 Axis in Progression of Esophageal Squamous Cell Carcinoma

INPP5A/HLA-G1/IL-10/MMP-21 Axis in Progression of Esophageal Squamous Cell Carcinoma

INPP5A/HLA-G1/IL-10/MMP-21 Axis in Progression of Esophageal Squamous Cell Carcinoma

Background: Background: Type I inositol polyphosphate-5-phosphatase A (INPP5A) is involved in different cellular events, including cell proliferation. Since INPP5A, HLAG1, IL-10, and matrix metalloproteinases (MMP)-21 genes play fundamental roles in esophageal squamous cell carcinoma (ESCC) tumorigenesis, we aimed in this study to clarify the possible interplay of these genes and explore the potential of these chemistries as a predictor marker for diagnosis in ESCC disease.

Methods: Methods: Gene expression analysis of INPP5A, HLAG-1, IL-10, and MMP-21 was performed using relative comparative real-time PCR in 56 ESCCs compared to their margin normal tissues. Immunohistochemical staining was accomplished for INPP5A in ESCCs. Analysis of ROC curves and the AUC were applied to evaluate the diagnostic capability of the candidate genes.

Results: Results: High levels of HLA-G1, MMP-21, and IL-10 were detected in nearly 23.2%, 62.5%, and 53.5% of ESCCs compared to the normal tissues, respectively, whereas INPP5A underexpression was detected in 19.6% of ESCCs, which all tested genes indicated significant correlations with each other. The protein expression level of INPP5A in ESCC tissues was significantly lower than that of the non-tumor esophageal tissues (p = 0.001). Interestingly, the concomitant expression of the INPP5A/HLA-G1, INPP5A/MMP-21, INPP5A/IL-10, HLA-G1/MMP-21, HLA-G1/IL-10, and MMP-21/IL-10 was significantly correlated with several clinicopathological variables. INPP5A, HLA-G1, MMP-21, and IL-10 showed to be the most appropriate candidates to discriminate tumor/non-tumor groups due to the total AUCs of all combinations (>60%).

Conclusion: Conclusion: Our results represent a new regulatory axis containing INPP5A/HLAG-1/IL-10/MMP-21 markers in ESCC development and may provide novel insight into the mechanism of immune evasion mediated by the INPP5A/HLAG-1/IL-10/MMP-21 regulatory network in the disease.

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来源期刊
Iranian Biomedical Journal
Iranian Biomedical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.20
自引率
0.00%
发文量
42
审稿时长
8 weeks
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