产低多分散、低分子量透明质酸的等价链球菌G群突变株的培育

Q2 Biochemistry, Genetics and Molecular Biology
Bahareh Jafari, Malihe Keramati, Reza Ahangari Cohan, Seyed Mohammad Atyabi, Sara Ali Hosseinzadeh
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引用次数: 1

摘要

背景:透明质酸(Hyaluronic acid, HA)是一种天然聚合物,在生物医药和化妆品领域有着广泛的应用,目前主要通过链球菌发酵生产。本研究采用化学随机诱变技术,培养了具有高HA产量的同种链球菌G群突变株。方法:方法:对野生菌株G组生产HA的最佳pH条件和培养时间进行评价。首先,用不同浓度的NTG诱变两轮。然后,在血液和透明质酸琼脂上筛选非溶血性和透明质酸酶阴性突变体。用咔唑法、琼脂糖凝胶电泳法和特异性染色法测定HA产率和分子量。并在10代内对高产突变体的稳定性进行了评价。结果:结果显示,野生型菌株在pH 5.5、培养4 h时产生1241±2.1µg/ml的HA,而筛选的突变体的HA产量提高了16.1-45.5%。两个突变株Gm2-120-21-3(2470±8.1µg/ml)和Gm2-120-21-4(2856±4.2µg/ml)滴度最高,产量一致。突变体的HA分子量(Mw)小于160 kDa,属于低分子量HA。结论:结论:该突变株具有低多分散、低分子量、高滴度的HA,经过进一步的安全性研究,可作为生产HA的潜在工业菌株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of Streptococcus equisimilis Group G Mutant Strains with Ability to Produce Low Polydisperse and Low-Molecular-Weight Hyaluronic Acid

Development of Streptococcus equisimilis Group G Mutant Strains with Ability to Produce Low Polydisperse and Low-Molecular-Weight Hyaluronic Acid

Development of Streptococcus equisimilis Group G Mutant Strains with Ability to Produce Low Polydisperse and Low-Molecular-Weight Hyaluronic Acid

Development of Streptococcus equisimilis Group G Mutant Strains with Ability to Produce Low Polydisperse and Low-Molecular-Weight Hyaluronic Acid

Background: Background: Hyaluronic acid (HA), a natural polymer with wide applications in biomedicine and cosmetics, is mainly produced by Streptococcal fermentation at industrial scale. In the present study, chemical random mutagenesis was used for development of Streptococcus equisimilis group G mutant strains with high HA productivity.

Methods: Methods: The optimum of the pH of culture condition and cultivation time for HA production by wild strain group G were assessed. At first, two rounds of mutation at different concentrations of NTG was used for mutagenesis. Then, the nonhemolytic and hyaluronidase-negative mutants were screened on the blood and HA agar. HA productivity and molecular weight were determined by carbazole assay, agarose gel electrophoresis and specific staining. Moreover, stability of the high producer mutants was evaluated within 10 generations.

Results: Results: The results showed that the wild-type strain produced 1241 ± 2.1 µg/ml of HA at pH 5.5 and 4 hours of cultivation, while the screened mutants showed a 16.1-45.5% increase in HA production. Two mutant strains, named Gm2-120-21-3 (2470 ± 8.1 µg/ml) and Gm2-120-21-4 (2856 ± 4.2 µg/ml), indicated the highest titer and a consistent production. The molecular weight (Mw) of HA for the mutants was less than 160 kDa, considering as a low Mw HA.

Conclusion: Conclusion: The mutant strains producing a low polydisperse, as well as low Mw of HA with high titer might be regarded as potential industrial strains for HA production after further safety investigations.

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来源期刊
Iranian Biomedical Journal
Iranian Biomedical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.20
自引率
0.00%
发文量
42
审稿时长
8 weeks
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